100 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(6) using phenolphthalein as indi- 

 cator. 

 (9) Acid and base solutions are to be 

 drawn into separately plugged bu- 

 rettes and allowed to stand several 

 hours to sterilize. 

 (10) Mix equal quantities of (6) and (7) 

 when ready for use. 



Sterilization: Method not specified. 



Use: As a general synthetic medium. 



Reference: Doryland (1916 pp. 146-148). 



356. Giltay and Aberson's Citrate Nitrate 

 Solution (Murray) 



Constituents : 



1. Water 1000.0 cc. 



2. KNO3 2.0 g. 



3. MgSO^ 2.0 g. 



4. Citric acid 5.0 g. 



5. K2HPO4 2.0 g. 



6. CaClz 0.20 g. 



7. NasCOa 4.25 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: To study denitrification under aerobic 



and anaerobic conditions. 

 Variants : 



(a) Fred used 0.2 g. K2HPO4 instead of 

 2.0 g. K2HPO4. 



(b) Fred used 2.0 g. NaNOs instead of 

 2.0 g. KNO3. 



(c) Christensen omitted the Na2C03, 

 used 3.0 g. of NaNOs instead of 

 KNO3, used 0.5 g. sodium citrate 

 instead of citric acid and added a 

 trace of iron chloride. 



(d) Harvey and Fercival omitted the 

 Na2C03, added a trace of FeCls and 

 used 2.0 g. of Na2N03 instead of 

 2.0 g. KNO3. 



References: Murray (1916 p. 611), Fred 

 (1911-12 p. 422), Christensen (1913 p. 421), 

 Harvey (1921-22 p. 108), Fercival (1920 

 p. 168). 



357. Percival's Tartrate Nitrate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Calcium tartrate 20.0 g. 



3. KNO3 20.0 g. 



4. K2HPO4 0.5 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 



Sterilization: Method not specified. 

 Use: To study denitrification. 

 Reference: Fercival (1920 p. 167). 



358. Gottheil's Nitrate Solution No. VII 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 1-0 g. 



3. CaCla 0.1 g. 



4. MgS04 0.3 g. 



5. NaCl 0.1 g. 



6. Iron trace 



7. KNO3 10.0 g. 



8. Sucrose 5.0 g. 



9. Glycerol 10.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 8 



and 9 in 1. 



Sterilization: Not specified. 



Use: General culture medium for organ- 

 isms found in the soil, on roots and on 

 rhizomes. 



Reference: Gottheil (1901 p. 432). 



359. Burri and Stutzer's Nitrate Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. KNO3 2.0 g. 



3. Glucose 2.0 g. 



4. MgS04 2.0 g! 



5. Citric acid 50 g. 



6. Potassium phosphate 2.0 g. 



7. CaCl2 0.2 g. 



8. FeCl, 2 drops 



Preparation : 



(1) Dissolve 2 and 3 in part of 1. 



(2) Dissolve 4, 5, 6, 7 and 8 in the re- 

 mainder of 1. 



(3) Boil (2) over a flame and neutralize 

 with soda. 



(4) Mix (1) and (3) and make up to 1 liter. 

 Sterilization: Sterilize in the steamer. 

 Use: To study nitrate reduction by B. 



denitrificans . 

 Variants : 



(a) Jensen used 2.0 g. NaNOj instead of 

 2.0 g. KNO3, specified the use of 

 K2HFO4 and omitted the FeCls. 



(b) V. Bazarewski used either 2.0 g. of 

 NaNO, or KNO3 and specified the 

 use of KH2FO4. 



Reference: Burri and Stutzer (1895 p. 393), 

 Jensen (1897 p. 623), v. Bazarewski 

 (1905 p. 4). 



