CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



101 



360. Kuntze's Nitrate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Salt peter 2.0 g. 



3. Glucose 2.0 g. 



4. MgS04 2.0 g. 



5. Citric acid 5.0 g. 



6. Potassium sulphate 2.0 g. 



7. CaCl2 0.2 g. 



8. Iron chloride 2 drops 



Preparation : 



(1) Dissolve 2 and 3 in part of 1. 



(2) Dissolve 4, 5, 6, 7 and 8 in the re- 

 mainder of 1. 



(3) During the heating of (2) neutralize 

 with soda. 



(4) Mi.x (3) and (1). 



C5) Distribute into 100.0 cc. lots in com- 

 bustion flasks. 

 (6) Inoculate with 5.0 g. manure. 



Sterilization: Method not given. 



Use: To study morphology and physiology 

 of denitrifying organisms, Bacillus deni- 

 trificnns agilis (Ampola and Garino) and 

 Bacillus oxalaticus (Zopf). 



Variants: Giltner gives the following solu- 

 tion: 



1. Distilled water 1000.0 g. 



2. KH2PO4 2.0 g. 



3. MgSOi 2.0 g. 



4. KNO3 1.0 g. 



5. CaCh 0.2 g. 



6. FeoCU solution 2.0 drops 



7. Citric acid 5.0 g. 



8. Glucose 10.0 g. 



References: Kuntze (1904 p. 2), Giltner 



(1921 p. 376). 



361. Giltay and Aberson's Nitrate Solution 



(Stoklasa and Vitek) 

 Constituents : 



1. Water 1000.0 cc. 



2. NaNOi 2.0 g. 



3. K2HPO4 2.0 g. 



4. CaCU 0.2 g. 



5. Glucose 2.0 g. 



6. Citric acid 5.0 g. 



7. Iron chloride few drops 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Neutralize 5.0 g. citric acid with soda. 



(3) Add (2) and several drops of iron 

 chloride to (1). 



Sterilization: Not specified. 



Use: To study nitrate reduction. 

 Reference: Stoklasa and Vitek (1905 

 p. 104). 



362. Giltay's Sucrose Nitrate Solution 

 (Fred) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NaNOa 2.0 g 



3. MgS04 2.0 g 



4. K2HPO4 2.0 g 



5. CaCla 0.2 g 



6. Citric acid 5.0 g 



7. NaoCOa 4.25 g 



8. Sucrose 2.0 g 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 



7 and 8 in 1. 



Sterilization: Not specified. 



Use: To study nitrate reduction by B. 

 fluorescens liquefaciens, B. pyocyaneus, 

 B. Hartlebi (H. Jensen), B. denitrificans . 



Reference: Fred (1911-12 p. 422). 



363. Behren's Cellulose Nitrate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. NH4NO3 10.0 g 



3. Sucrose 2.0 g 



4. Potassium phosphate 5.0 g 



5. MgS04 1.0 g 



6. Lactic acid 2.0 g 



7. Swedish filter paper 

 Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Filter. 



(3) Distribute in 100.0 cc. lots. 



(4) Add pieces of purified Swedish filter 

 paper to each flask. 



Sterilization: Not specified. 



Use: To study utilization of cellulose by 

 Botrylis vulgaris, Pencillium glaucum 

 and Penicillium luteum. The author 

 reported that Botrylis vulgaris dissolved 

 the cellulose. 



Reference: Behrens (1898 p. 549). 



364. Maassen's Malic Acid Nitrate Solution 

 (Stoklasa and Vitek) 



Constituents: 



1. Water 1000.0 cc. 



2. NaCl 0.5 g. 



3. Na.COa 0.5 g. 



4. MgS04 0.1 g. 



5. Malic acid 7.0 g. 



