102 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



6. Glycerol 20.0 g. 



7. NaNOa 2.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Neutralize 7.0 g. malic acid with 

 NajCOa. 



(3) Add (2), 6 and 7 to (1). 

 Sterilization: Not specified. 

 Use: To study nitrate reduction. 

 Reference: Stoklasa and Vitek (1905 



p. 104). 



365. Maassen's Stearate Nitrate Solution 

 (Vierling) 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerin 20.0 g. 



3. KNO, 2.5 g. 



4. Potassium stearate 1-0 g. 



5. MgS04 1.0 g. 



6. K2HPO4 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization: Not specified. 



Use: To study the reduction of nitrate by 

 Mycobacteria. Author reported that 

 little or no ammonia was produced. 



Reference: Vierling (1920 p. 202). 



SUBGROUP I-C. SECTION 5 



Liquid media or basal solutions with con- 

 stituents of known chemical composition; 

 organic nitrogen present as amino acids; 

 other forms of organic nitrogen may also 

 be present. 



Ai.* Only one amino acid present. 

 Bi.t Incomplete or basal solutions, used 



with the addition of a variety of other 



nutrients. 

 Ci. Amino nitrogen supplied as glycocoll. 



Went's Basal Glycocoll Solution 366 



Wherry's Basal Glycocoll Solution. . . 367 



Waksman's Basal Glycerol Glycocoll 



Solution 368 



C2. Amino nitrogen supplied as alanine. 



Went's Basal Alanin Solution 369 



C3. Amino nitrogen supplied as tyrosine. 



Went's Basal Tyrosine Solution 370 



C4. Amino nitrogen supplied as leucine. 



Waksman's Basal Glycerol Leucine 

 Solution 371 



' See page 104 for A2. 

 See bottom of next column for B2. 



Went's Basal Leucine Solution 372 



Cb. Amino nitrogen supplied as aspartic 



acid or asparagin. 

 Di. Containing no other added organic 



carbon. 



van Delden's Basal Asparagin Salt 

 Solution 373 



Uschinsky's Basal Asparagin Solution 

 (Kendall, Day and Walker) 374 



Kita's Basal Asparagin Solution 375 



Proskauer and Beck's Basal Aspara- 

 gin Solution 376 



Hefferan's Basal Asparagin Solution. 377 



Zikes' Basal Asparagin Solution 378 



Miehe's Basal Asparagin Solution. . . 379 



Gottheil's Basal Asparagin Solution. 380 



Went's Basal Asparagin Solution 381 



Sullivan's Basal Asparagin Solution. 382 

 D2. Containing additional organic carbon. 

 El. Only one type of organic carbon added. 

 Fi. Carbohydrates, only, added. 



Munter's Basal Glucose Asparagin 

 Solution 383 



Ono's Basal Sucrose Asparagin Solu- 

 tion 384 



Bokorny's Basal Asparagin Solution. 385 



Higgins' Basal Carbohydrate Aspara- 

 gin Solution 386 



F2. Alcohols only added. 



Peklo's Basal Asparagin Solution... 387 



Sullivan's Basal Glycerol Asparagin 

 Solution 388 



Waksman's Basal Glycerol Asparagin 



Solution 389 



F3. Acids or their salts, only, added. 



Tanner's Basal Lactate Asparagin 

 Solution 390 



Hiss' Basal Litmus Asparagin Solu- 

 tion 391 



Harvey's Basal Lactate Asparagin 

 Solution 392 



Capaldi and Proskauer' s Basal Ci- 

 trate Asparagin Solution 393 



Mendel's Basal Citrate Asparagin 

 Solution 394 



Maassen's Basal Malic Acid Aspara- 

 gin Solution (Smith) 395 



E2. More than one type of organic carbon 



added. 



Lockemann's Basal Citrate Glycerol 

 Solution 396 



Mendel's Basal Citrate Glycerol 



Solution 397 



B2. Complete media. 



