CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



105 



Preparation : 



(1) Prepare 5.0% solutions of one of the 

 materials listed in added nutrients. 



(2) Flask in 25.0 cc. quantities. 



(3) Prepare a 0.66% solution of glycocoll. 



(4) Add 25.0 cc. of (3) to each flask of (2). 

 Sterilization: Method not given. 



Use: To study nutrients for Monilia sito- 

 phila (Mont.) Sacc. The materials 

 ranked as follows as suitable additional 

 carbon sources: maltose, glucose, lactose, 

 sucrose and glycerol. 

 Added nutrients: The author used the 

 following additional carbon sources: 

 maltose glucose 



lactose glycerol 



sucrose 

 Variants: The author used the basic solu- 

 tion without additional materials. 

 Reference: Went (1901 p. 593). 



367 Wherry's Basal Glycocoll Solution 



Constituents: 



1. Redistilled water 



2. NaCl 



3. KCl 



4. CaClj 



5. MgSOi 



6. Glycocoll 



7. (NH4)2HP04 



(Salts to be Kahlbaum's C.P.) 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Add one of the added nutrients. 



(3) Adjustment of reaction not given. 

 Sterilization: Sterilize in autoclave, time 



not given. 

 Use: To study morphology of B. tubercu- 

 losis. Growth appeared as very thin 

 scattered pellicles composed of non-acid- 

 proof coccoid bodies and thin segmented 

 rods. 

 Added nutrients: The author added one 

 of the following: 

 (NHOaHPO^ 2.0 g. 



r(NH4)2HP04 2.0 g. 



\(NH4)2S04 2.0 g. 



rKH2P04 2.0 g. 



\NH4Cl 2.0 g. 



fKH2P04 2.0 g. 



\(NH4)2S04 2.0 g. 



Reference: Wherry (1913 p. 152). 



368. Waksman's Basal Glycerol Glycocoll 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Glycerol 30.0 g. 



3. KokPOi 1.0 g. 



4. KCl 0.5 g. 



5. MgS04 0.5 g. 



6. FeS04 0.01 g. 



7. Glycocoll 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 and 2.0 g. 

 of one of the added nutrients in 1. 



(2) Tube in 10-12 cc. lots. 



(3) Adjustment of reaction not specified. 

 Sterilization: Sterilize at 15 pounds for 



15 minutes. 

 Use: To study metabolism of actinomy- 



cetes. 

 Added nutrients: The author added 2.0 g. 

 of one of the following: 



NaXOs (NH 4)280 4 



XaX02 (XH 4)2003 



Reference: Waksman (1920 p. 3). 



369. Went's Basal Alanin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Alanin (0.66%) 6.6 g. 



Preparation : 



(1) Prepare 5.0% solutions of one of the 

 materials listed in added nutrients. 



(2) Flask in 25.0 cc. quantities. 



(3) Prepare a 0.66% solution of alanin. 



(4) Add 25.0 cc. of (3) to each flask of (2). 

 Sterilization: Method not given. 



Use: To study the nutrients for Monilia 

 sitophila (Mont.) Sacc. The materials 

 ranked as follows as suitable carbon 

 sources with alanin as a nitrogen source: 

 glycerol, sucrose, maltose, lactose and 

 glucose. 

 Added nutrients: The author used the 

 following additional carbon sources: 

 maltose glucose 



lactose glycerol 



sucrose 

 Variants: The author used the basal solu- 

 tion without additional materials. 

 Reference: Went (1901 p. 593). 



