106 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



370. Went's Basal Tyrosine Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Tyrosine (0.66%) 6.6 g. 



Preparation : 



(1) Prepare 5.0% solutions of one of the 

 materials listed in added nutrients. 



(2) Flask in 25.0 cc. quantities. 



(3) Prepare a 0.66% tyrosine solution. 



(4) Add 25.0 cc. of (3) to each flask of (2). 

 Sterilization: Method not given. 



Use: To study nutrients for Monilia sito- 

 phila (Mont.) Sacc. The materials 

 ranked as follows as suitable carbon 

 sources with tyrosine as a nitrogen source : 

 maltose, glucose, sucrose, lactose, glyc- 

 erol. 

 Added nutrients: The author used the 

 following additional carbon sources: 

 maltose glucose 



lactose glycerol 



sucrose 

 Variants: The author used the basic solu- 

 tion without additional materials. 

 Reference: Went (1901 p. 593). 



371. Waksman's Basal Glycerol Leucine 



Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Glycerol 30.0 g. 



3. K2HPO4 1.0 g. 



4. KCl 0.5 g. 



5. MgS04 0.5 g. 



6. reS04 0.01 g. 



7. Leucine 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 2.0 g. of 

 one of the added nutrients in 1. 



(2) Tube in 10-12 cc. lots. 



(3) Adjustment of reaction not specified. 

 Sterilization: Sterilize at 15 pounds for 15 



minutes. 

 Use: To study metabolism of actinomy- 



cetes. 

 Added nutrients: The author added 2.0 g. 

 of one of the following: 



NaXOs (NH 4)280 4 



NaNOs (NH4)2C03 



Reference: Waksman (1920 p. 3). 



372. Went's Basal Leucine Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Leucine (0.66%) 6.6 g. 



Preparation : 



(1) Prepare 5.0% solutions of one of the 

 materials listed in added nutrients. 



(2) Flask in 25.0 cc. quantities. 



(3) Prepare a 0.66% solution of leucine. 



(4) Add 25.0 cc. of (3) to each flask of (2). 

 Sterilization: Method not given. 



Use: To study the nutrients for Monilia 

 sitophila (Mont.) Sacc. The materials 

 ranked as follows as suitable carbon 

 sources with leucine as nitrogen source: 

 glucose and maltose, sucrose, glycerol, 

 lactose. 

 Added nutrients: The author used the 

 following additional carbon sources: 

 maltose glucose 



lactose glycerol 



sucrose 

 Variants: The author used the basic solu- 

 tion without additional materials. 

 Reference: Went (1901 p. 593). 



373. van Delden's Basal Asparagin Salt 

 Solution 



Constituents : 



1. Water, tap 1000.0 cc, 



2. K2HPO4 0.5 g. 



3. Asparagin 5.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Additional nutrients are added as 

 indicated below. 



Sterilization: Not specified. 



Use: To study sulphate reduction by 



Microspira desidfuricans. 

 Added nutrients and variants : The author 



employed the following solutions: 



(a) Added 2.0 g. MgS04-7H20. 



(b) 5.0 g. potassium succinate, 5.0 g. 

 gypsum and 1.0 g. of asparagin. 



(c) 5.0 g. potassium citrate, 5.0 g. gyp- 

 sum and 1.0 g. of asparagin. 



(d) Specified the use of sea water in the 

 preparation of the basic solution, 

 used 1.0 g. of asparagin and added 

 5.0 g. of sodium lactate or 2.0 g. 

 glucose. 



(e) 1.0 g. asparagin in the basic solution 

 and added 30.0 g. NaCl, and 8.0 g. 

 MgS04-7H20. To this solution he 

 added 0.0, 5.0 or 10.0 g. of sodium 

 lactate. 



(f) Added 30.0 g. NaCl and 12.0 g. of 

 gypsum to the basic solution. 



