CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



107 



(g) Added 10.0 g. glucose, 30.0 g. NaCl 

 and 8.0 g. MgSOi-THaO to the basic 

 solution. 



(h) Used 2.5 g. asparagin in the basic 

 solution and added 30.0 g. NaCl, 

 8.0 g. MgS04-7H20 and 5.0 g. sodium 

 lactate. 



(i) Used 1.0 g. asparagin in the basic 

 solution, added 8.0 g. MgS04-7H20, 

 30.0 g. NaCl and 5.0 ^g. of sodium 

 lactate or potassium succinate. 



(j) Used 1.0 g. of asparagin in the basic 



solution and added 5.0 g. sodium 



lactate, 75.0 g. NaCl and 8.0 g. 



MgS04-7H20. 



Reference: van Delden (1903-04 p. 85, 105). 



374. Uschinsky's Basal Asparagin Solution 

 (Kendall, Day and Walker) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Asparagin 4.0 g. 



3. NasHPOi 2.0 g. 



4. NaCl 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and one of the ma 4- 

 terials listed under added nutrients 

 in 1. 



(2) Distribute in 100.0 cc. lots. 



(3) Adjustment of reaction not given. 

 Sterilization: Method not given. 



Use: To study metabolism by the tubercle 

 bacilli. Authors used alizarin, neutral 

 red and phenolphthalein to study changes 

 in reaction; Ziehl-Neelsen stain for 

 staining. 

 Added nutrients: The following organic 

 materials were added: 



glucose 10.0 g. 



mannitol 10.0 g. 



glycerol 30.0 g. 



The following materials were added to 

 study lipase production: different es- 

 ters were incubated for 24 hours with 

 clear bacteria free culture broth, and 

 amount of acid produced, measured in 

 terms of n/50 NaOH, determined lipase 

 production, 

 ethyl alcohol and (NH4)2HP04 amounts 



not given, 

 glycerol and (NH4)2HP04 amounts not 



given, 

 glucose and (NH4)2HP04 amounts not 

 given. 



References: Kendall, Day and Walker 

 (1914 p. 428), Kendall, Walker and Day 

 (1914 p. 355). 



375. Kita's Basal Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. MgS04 2.5 g. 



3. KH2PO4 5.0 g. 



4. FeCh solution drops 



5. Asparagin 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 5.0% of any carbohydrate. 

 Sterilization: Not specified. 



Use: Cultivation of Japanese molds, Asper- 

 gillus Okazaki, Aspergillus candidus, 

 Aspergillus albus, Aspergillus tamarii, 

 Pseudorhizopus, Aspergillus glaucum. 



Added nutrients: Add 5.0% of any carbo- 

 hydrate. 



Variants : 



(a) Buromsky used the following solution 

 to study effect of organic acids on 

 yeast, Saccharomyces ellipsoid, Sac- 

 charomyces pastorianus. 



1. Water 1000.0 g. 



2. MgSOi 0.5 g. 



3. KH2PO4 1.0 g. 



4. Asparagin 5.0 g. 



The solution was prepared by 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Distribute in 100.0 cc. lots in 500.0 

 cc. Erlenmeyer flasks. 



(3) Add one of the listed materials 

 below as additional carbon source. 



(4) Sterilize on 3 successive days in a 

 Koch steamer, 30 minutes the first 

 day, and 20 minutes on each the 

 2nd and 3rd day, (a slight turbidity 

 develops when glycerin or mannite 

 is added). 



The following nutrients were added: 



quinic acid 0.5% 



citric acid 1-0% 



tartaric acid 1-0% 



succinic acid 1-0% 



glycerol 1.0% 



mannitol 1 .0% 



(b) von Bronsart used 0.25% KH2PO4 

 and 5.0% asparagin instead of 0.1% 

 KH2PO4 and 0.5% asparagin. He 

 added one of the following carbon 

 sources (amount not specified). 



