108 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



starch levulose 



dextrin glucose 



sucrose 

 He used the solution for the cultivation 

 of Xylaria, Xylaria hypoxylon. 

 References: Kita (1913 p. 434), Buromsky 

 (1914 p. 532), von Bronsart (1919 p. 59). 



376. Proskauer and Beck's Basal Asparagin 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Potassium phosphate 



(0.5%) 5.0g. 



3. MgSO4(0.25%) 2.5 g. 



4. Asparagin (0.5%) 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the combinations listed 

 under added nutrients. 



Sterilization: Not specified. 



Use: To study the constituents essential 



for the growth of tubercle bacilli. 

 Added nutrients : The authors added one of 



the following combinations: 



(a) citric acid 0.075% 



glycerol 4.0% 



glucose 1.0% 



K2SO4 0.25% 



NaCl 0.15% 



(b) citric acid 0.75% 



glycerol 4.0% 



K2SO4 0.25% 



NaCl 0.15% 



(c) Glycerol 4.0% 



glucose 1.0 or 0.0% 



K2SO4 0.25% 



NaCl 0.15% 



(d) Glycerol 1.5% 



K2SO4 0.25% 



NaCl 0.15% 



(e) Glycerol 1.0% 



K2SO4 0.25% 



NaCl 0.15% 



(f) Glycerol 1.0% 



One of the following: 



citric acid 1-0% 



lactic acid 0.1% 



tartaric acid 0.75% 



malic acid 0.75% 



succinic acid 0.75% 



aspartic acid 0.75% 



alanin 0.75% 



(g) Glycerol 1.0% 



citric acid 0.1% 



alanin 0.1% 



(h) citric acid 1-5% 



One of the following: 



alanin 0.1% 



fumaric acid 0.075% 



aconitic acid 0.075% 



citraconic acid 0.075% 



itaconic acid 0.075% 



(i) citric acid 1.0% 



glyceric acid 1-0% 



or 



Biuret 0.075% 



Variants : 



(a) The authors used 0.3% potassium 

 phosphate and added 0.25% mag- 

 nesium citrate and 1.5% citric acid. 



(b) The authors used 0.06% MgS04 and 

 added 0.25% magnesium citrate and 

 1.5% citric acid. 



(c) The authors used 0.1, 0.2, 0.3, 0.4, 

 0.5 or 0.6% asparagin without addi- 

 tions. 



Reference: Proskauer and Beck (1894 

 p. 139). 



377. Hefferan's Basal Asparagin Solution 



Constituents : 



1. Redistilled water 1000.0 cc. 



2. Asparagin 2.0 g. 



Preparation : 



(1) Dissolve 2 in redistilled water. 



(2) Reaction is neutral to phenol- 

 phthalein. 



(3) Distribute in Jena flasks that have 

 been cleaned in acid and rinsed re- 

 peatedly in distilled water. 



(4) Add one of the combinations given 

 below. 



Sterilization: Not specified. 



Use: To study pigment production by red 

 pigment forming bacteria, B. prodigiosus, 

 B. ruberbalticus, B. kiliensis, B. ruber- 

 miquel, B. rutilus, B. amyloruber. Pig- 

 ment production generally poor. 



Added nutrients : One of the following ma- 

 terials or combinations may be added; 



(a) 1.0 g. MgS04 and 1.0 g. K2HPO4. 



(b) 1.0 g. K2HPO4. 



(c) 1.0 g. MgS04. 



(d) 1.0 g. MgS04, 1.0 g. K2HPO4 and 

 20.0 g. glycerol. 



