CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



109 



(e) 1.0 g. MgS04, 1.0 g. K2HPO4 and 

 10.0 g. glucose. 



(f) 1.0 g. MgS04, 1.0 g. K2HPO4 and 

 10.0 g. lactose. 



(g) 1.0 g. MgS04, 1.0 g. K.HPO^ and 

 10.0 g. sucrose. 



Reference: Hefferan (1903-4 p. 522). 



378. Zikes Basal Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Asparagin 2.5 g. 



3. K2HPO4 1.0 g. 



4. MgS04 0.3 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add 10.0% of one of the carbon 

 sources listed under added nutrients. 



Sterilization: Not specified. 



Use: Volutin production by yeast, Oidium 

 lactis. 



Added nutrients: The author used 10.0% 

 of one of the following carbon sources: 

 glucose lactose 



fructose raffinose 



galactose dextrin 



maltose inulin 



sucrose 



Reference: Zikes (1922 p. 34). 



379. Miehe's Basal Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 (0.1%) 1.0 g. 



3. MgS04 (0.02%) 0.2 g. 



4. CaCl2 (0.01%) 0.1 g. 



5. Asparagin (0.5%) 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 4.0% of one of the added nu- 

 trients. 



Sterilization: Not specified. 



Use: To study carbon sources suitable for 

 tubercle bacilli. 



Added variants: The following materials 

 were added: 



glycerol 4.0% 



dextrin 4.0% 



glucose 4.0% 



maltose 4.0% 



xylose 4.0% 



Reference: Miehe (1908-9 p. 137). 



380. Gottheil's Basal Asparagin Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 1-0 g. 



3. CaClj 0.1 g. 



4. MgS04 0.3 g. 



5. NaCl 0.1 g. 



6. Ifon trace 



7. Asparagin 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Add one of the added nutrients in 

 the amount indicated. 



Sterilization: Not specified. 



Use: Cultivation of organisms found in the 

 soil, on roots and on rhizomes. 



Added nutrients : One of the following com- 

 binations were added: 



(a) glycerol 10.0 g. 



sucrose 5.0 g. 



(b) sucrose 5.0 g. 



(c) galactose 3.0 g. 



lactose 3.0 g. 



(d) galactose 3.0 g. 



glycerol 3.0 g. 



Reference: Gottheil (1901 p. 432). 



381. Went's Basal Asparagin Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Asparagin (0.66%) 6.6 g. 



Preparation : 



(1) Prepare 5.0% solutions of one of the 

 materials listed in added nutrients. 



(2) Flask in 25.0 cc. quantities. 



(3) Prepare a 0.66% solution of asparagin. 



(4) Add 25.0 cc. of (3) to each flask of (2). 

 Sterilization: Method not given. 



Use: To study the nutrients for Monilia 

 sitophila (Mont.) Sacc. The materials 

 ranked as follows as suitable carbon 

 sources, with nitrogen supplied as aspara- 

 gin: sucrose, glycerol, glucose, maltose 

 and lactose. 

 Added nutrients: The author used the 

 following additional carbon sources: 

 maltose glucose 



lactose glycerol 



sucrose 

 Variants: The author used the basic solu- 

 tion without additional materials. 

 Reference: Went (1901 p. 593). 



