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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



382. Sullivan's Basal Asparagin Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Asparagin 10.0 g. 



Preparation : 



(1) Dissolve 2 in 1. 



(2) Add one of the materials listed under 

 added nutrients. 



Sterilization: Not specified. 



Use: To study pigment formation. The 

 author reported that the blue-green 

 pyocyanin was formed whenever there 

 was present a suitable combination of 

 carbon, hydrogen, oxygen, and nitrogen 

 and some salts to aid synthesis. Its 

 production was independent of either a 

 phosphate or a sulphate. 



Added nutrients: 



fMgS04 2.0 g. 



(a) K2HPO4 1.0 g. 



[NasS 0.2 g. 



(b) MgS04 0.2 g. 



. fK,HP04 l.Og. 



^''^ \Na2SO4 l.Og. 



, [MgSO^ 0.2 g. 



^""^ IK2HPO4 l.Og. 



. . fMgS04 0.01 to l.Og. 



^*''' \K2HPO4 l.Og. 



.J. fMgS04 1.0 to 16.0 g. 



[peptone 10.0 g. 



fMgS04 0.2 g. 



^^^ \K2HPO4 0.01 to 5.0 g. 



[MgS04 0.2 g. 



(h) K2HPO4 l.Og. 



[peptone 5.0 g. 



Variants: The author used 5.0 g. asparagin 

 and added 0.2 g. MgS04, 1.0 g. K2HPO4, 

 and 4.0 g. ammonium lactate. 

 Reference: Sullivan (1905-06 pp. 127-140). 



383. Miinter's Basal Glucose Asparagin 

 Solution 

 Constituents: 



1- Water 1000.0 cc. 



2. NaCl 0.5 g. 



3. MgS04 0.5 g. 



4. CaCl2 Olg 



5. K2HPO4 1.5 g. 



6. NH4NO3 l.Og. 



7. Asparagin 0.5 g. 



8. Dextrose 9-0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Neutralize with NasCOj. 



(3) Distribute in 50.0 cc. lots in small 

 Erlenmeyer flasks. 



(4) Add one of the added nutrients. 

 Sterilization: Method not given. 



Use: Cultivation of Actinomyces odorifer, 

 Act. chromogenes, Act. albus I and II, 

 Act. S. a, b, andc. The author reported 

 that the actinomycetes preferred a 

 neutral medium. They tolerated a very 

 small amount of acid however. No 

 growth in highly acid solutions. 



Added nutrients: Add one of the following 

 to each flask: 

 acetic acid 0.01% or 0.1%. 

 succinic acid 0.004%, 0.04% or 0.1%. 

 malic acid 0.004%, 0.01% or 0.1%. 

 aspartic acid 0.01%, 0.05%, 0.1%, 0.2% 

 or 0.4%. 



Variants: The author used the basic solu- 

 tion without any additions. 



Reference: Mlinter (1913 p. 378). 



384. Ono's Basal Sucrose Asparagin 

 Solution 

 Constituents : 



1. Water 1000.0 g. 



2. Sucrose 85.0 g. 



3. Asparagin 16.0 g. 



4. MgS04 3.0 g. 



5. KH2PO4 5.0 g. 



6. FeS04 trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Distribute in 30.0 cc. lots. 

 Sterilization: Sterilize in the steamer. 

 Use: To study the effect of small amounts 



of chemicals on the growth of Aspergillus 

 niger. The author reported that small 

 amounts of CUSO4 tended to increase 

 growth while concentrations higher than 

 1/250 molar CUSO4 tended to hinder 

 growth. 

 Variants and added nutrients: 



(a) The author added CUSO4 solution so 

 that the medium was from 1/32,000 to 

 1/250 molar CUSO4. 



(b) Henneberg used 0.3% asparagin, 0.2% 

 MgS04, 5.0% sucrose and omitted the 

 FeS04. To this solution he added 

 the following materials : 



NaCl 0.1% 



CaCl2 0.1% 



K2SO4 0.1% 



