CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



111 



Iron sulphate 0.01% 



Calcium lactate 10% 



gypsum 0.1% 



K2CO, 0.5% 



soda 0.1 to 0.6% 



MgCOa 0.1 to 1.0% 



(c) Henneberg used 3.0 to 6.0% aspara- 

 gin, 10.0 to 15.0% sucrose, 0.2% 

 MgSO* and omitted the FeS04 from 

 the basic solution. To this solution 

 he added one of the following: 



dextrin 1.0% 



ammonium tartrate 0.3% 



peptone 0.5% 



potassium phosphate 0.5% 



K2HPO4 0.5% 



[peptone 0.5% 



ICaClj 0.1% 



fCaClj 0.1% 



\Chalk 



(d) Henneberg used 0.3% asparagin, 

 15.0% sucrose, 0.2% MgS04 and 

 specified the use of either KH2PO4 

 or K2HPO4. To this solution he sug- 

 gested that one of the following might 

 be added : 



gypsum 2.0% 



potassium phosphate 2.0% 



CaCla 0.6% 



potassium lactate 2.0% 



K2CO3 2.0% 



soda 0.6% 



K2SO4 0.6% 



(e) Bokorny used a medium containing 

 0.5% asparagin, 0.03% MgS04, a 

 trace of CaCl2, 10.0% sucrose and 

 added one of the following: 



K2HPO4 0.1, 0.5, 1.0, 2.0 or 4.0% 

 KH2PO4 0.1, 0.5, 1.0, 2.0 or 4.0% 

 NajHPOi 0.1, 0.5, 1.0, 2.0 or 4.0% 

 KH2PO4 0.1% with NaCl 0.5, 1.0, 2.0 or 



4.0% 

 KH2PO4 0.1% with KI 0.01, 0.05, 0.1 or 



0.5% 

 KH2PO4 0.1% with KBr 0.01, 0.05, 0.1 



or 0.5% 

 KH2PO4 0.1% with NasCOs 0.5 or 1.0% 

 KH2PO4 0.1% with NaF 0.05, 0.1 or 



0.5% 

 KH2PO4 0.1% with Na2S203 0.5 or 1.0% 

 He reported that 4.0% K2HPO4, 

 KH2PO4 or Na2HP04 did not inhibit 

 the growth of yeast, 2 and 4.0% NaCl 

 inhibited growth. Generally KI and 



KBr were toxic for yeast. Na2C03 

 inhibited growth. NaF inhibited 

 growth of yeast. A heavier growth of 

 yeast appeared on medium contain- 

 ing only 0.5% Na2S203. 



(f) Bokorny used a medium containing 

 1.0% sucrose, 0.1% asparagin, 0.1% 

 KH2PO4, 0.05% MgS04 and a trace 

 of iron chloride. He reported that 

 yeast would grow more luxuriantly 

 if the medium contained 0.05% CaCl2. 

 Calcium was thought to be a necessity 

 for growth. Growth occurred in the 

 solution without calcium due to the 

 probable presence of calcium in the 

 sucrose or asparagin as an impurity. 



(g) Bokorny reported that spirogyra 

 grew much more luxuriant and more 

 characteristic in the following me- 

 dium when 0.05% CaCl2 was added : 



1. Water 1000.0 g. 



2. Sucrose 10.0 g. 



3. Asparagin 2.5 g. 



4. KH2PO4 0.5 g. 



5. MgS04 0.5 g. 



6. CaCls 0.0 or 0.5 g. 



(h) Zikes used the following solution to 



study perithecium formation by 

 Aspergillus oryzae: 



1. Water 1000.0 g. 



2. Asparagin 10.0 g. 



3. K2HPO4 5.0 g. 



4. MgS04 2.5 g. 



5. Sucrose 75.0 g. 



(i) Zikes used the following solution to 

 study volutin production by a num- 

 ber of wine and other yeasts: 



1. Water, distilled 1000.0 cc. 



2. Sucrose 100.0 g. 



3. Asparagin 2.5 g. 



4. K2HPO4 1.0 g. 



5. MgS04 0.3 g. 



pH = 5.0706, CH = 8.50 X 10-« 



References: Ono (1902 p. 155), Henneberg 

 (1907 pp. 40-45), (1909 pp. 104, 105), 

 Bokorny (1912 pp. 119, 120, 135, 138, 144, 

 147), Zikes (1922 pp. 29, 340). 



385. Bokorny's Basal Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Sucrose 100.0 g. 



3. Asparagin (0.5%) 5.0 g, 



4. KH2PO4 (0.1%) 1.0 g. 



