112 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



5. MgSOi (0.03%) 0.3 g. 



6. CaCl2 trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add one of the salts given in added 

 nutrients. 



Sterilization: Not specified. 



Use: To study the effect of metallic salts 



on development of yeast. 

 Added nutrients: The following salts were 



added: 



CaS04 0.1 or 0.02 g. 



MnS04 1.0, 3.0 or 5.0 g. 



KMn04 0.1, 0.05 or 0.01 g. 



NiS04 0.02, 0.03 or 01 g. 



KzCrOi (red 1/3 neutral) 0.1, 0.05 or 0.5 g. 



K2Cr04 (yellow) 0.1 or 0.001 g. 



Co(NO3)3 0.1, 0.02 or 0.2 g. 



ZnCls 0.2, 0.1, 0.05, 0.025 or 0.01 g. 



ZnSO4 0.1, 0.5 or 1.0 g. 



perosmic acid Os04 0.05, 0.01, 0.005, 0.5 

 or 0.1 g. 



Author omitted the trace of CaCl2. 

 Reference: Bokorny (1921 pp. 153-182). 



386. Higgins' Basal Carbohydrate Asparagin 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Asparagin 4.0 g. 



3. Lactose 2.5 g. 



4. Glucose 2.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the combinations given 

 under added nutrients. 



(3) Adjust to neutral to phenolphthalein. 

 Sterilization: Method not given. 



Use: Cultivation of organisms causing 

 cholera and Picaton cattle disease. 



Added nutrients: The following combina- 

 tions were added: 



(a) Potassium phosphate 2.0 g 



KNO3 4.0 g 



(b) Ammonium succinate 4.0 g 



potassium phosphate 2.0 g 



K2SO4 1.0 g 



NH4CI 1.5 g 



(c) Ammonium phosphate 2.5 g 



KNO3 2.5 g 



KCl 1.5 g 



(NH4)2S04 l.Og 



Reference: Higgins (1898 pp. 666-667). 



387. Peklo's Basal Asparagin Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. KH2PO4 10.0 g. 



3. CaClj l.Og. 



4. MgS04 3.0 g. 



5. NaCl l.Og. 



6. Fe2Cl6 (Merck siccum) trace 



7. Mannitol 20.0 g. 



8. Asparagin 4.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add one of the added nutrients. 

 Sterilization: Generally sterilize all solu- 

 tions for 30 minutes (method not given). 



Use: Cultivation of plant actinomyces. 

 Added nutrients with modifications: The 

 following combinations were added: 



(a) The author used 1.0 g. KH2PO4, 0.1 g. 

 CaCl2, 0.3 g. MgS04 and 0.1 g. 

 NaCl and added 20.0 g. mannitol 

 and 4.0 g. asparagin. The reaction 

 was acid before sterilization. May 

 be neutralized by the addition of 

 Na2C03. 



(b) The author used 7.5 g. KH2PO4, and 

 added 20.0 g. of mannitol and 4.0 g. 

 asparagin. The reaction was slightly 

 acid before sterilization. May be 

 neutralized by the addition of 

 NaaCOs. 



(c) Add 100.0 cc. of the basal solution to 

 900.0 cc. of distilled water, and then 

 add 20.0 g. mannitol and 4.0 g. 

 asparagin. The reaction was weakly 

 acid. The author specified that the 

 solution might be neutralized by the 

 addition of Na2C03. 



(d) Mi.x 250.0 cc. of the basal solution and 

 add 750.0 cc. of distilled water, and 

 add 20.0 g. mannitol and 4.0 g. 

 asparagin. The action was distinctly 

 acid. 



(e) Mi.x 100.0 cc. of the basal solution 

 with 900.0 cc. of distilled water. Add 

 30.0 g. mannitol and 4.0 g. asparagin. 

 The reaction was weakly acid. 



(f) Mix 250.0 cc. of the basal solution 

 with 750.0 cc. of distilled water. Add 

 30.0 g. mannitol and 4.0 g. asparagin. 

 The reaction was distinctly acid. 



(g) The author used 5.0 g. KH2PO4 and 

 0.5 g. CaCU and added 5.0 g. K2HPO4 

 to the basal solution. To this solu- 



