CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



113 



tion 20.0 g. mannitol and 4.0 g. aspara- 

 gin were added. Reaction was dis- 

 tinctly acid. Filter the precipitate 

 after the first sterilization. Sterilize 

 for 90 minutes all together, 

 (h) The author used 5.0 g. KH2PO4 and 

 0.5 g. CaCl2 and added 5.0 g. K2HPO4 

 to the basal solution. 100.0 cc. of 

 this solution was diluted with 900.0 

 cc. of water, and 20.0 g. mannitol and 

 4.0 g. asparagin were added. Reac- 

 tion was weakly acid. Filter the 

 precipitate after the first steriliza- 

 tion. Sterilize for 90 minutes all 

 together, 

 (i) The author used 5.0 g. KH.PO4, and 

 0.5 g. CaCl2, omitted the MgSOi and 

 NaCl from the basal solution and 

 added 5.0 g. K2HPO4 and 3.0 g. 

 MgS04. To 200.0 cc. of this solution 

 added 800.0 cc. distilled water, 20.0 g. 

 mannitol and 4.0 g. asparagin. The 

 reaction is weakly acid. Sterilize 

 for one hour. 

 Reference: Peklo (1910 p. 470). 



388. Sullivan's Basal Glycerol Asparagin 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol 10.0 g. 



3. Asparagin 2.0 g. 



4. K2HPO4 1.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add the various materials as indi- 

 cated under added nutrients. 



Sterilization: Not specified. 



Use: To study pigment formation. The 

 author reported that the blue-green 

 pyoc3'anin was formed whenever there 

 was present suitable combination of car- 

 bon hydrogen, oxygen and nitrogen and 

 some salts to aid synthesis. For the 

 formation of the fluorescent pigment, 

 the presence of both sulphur and phos- 

 phorus was essential. 



Added nutrients and variants: 



(a) The author added 0.2 g. of MgSOi to 

 the basal solution. Most chromo- 

 genic bacteria produced a pigment on 

 this medium. 



(b) Substituted 5.0 g. of asparagin for 

 2.0 g. 



(c) Added 1.0 g. KXO,. 



(d) Used 20.0 g. glycerol, 3.0 g. asparagin 

 and added 1.0 g. XaCl. 



(e) Omitted the K2HPO4, used 10 to 

 20.0 g. glycerol, 10.0 g. asparagin, 

 and added 0.2 to 1.0 g. KNO3 and 

 0.2 to 1.0 g. XaCl. 



(f) Used 10 to 20.0 g. glycerol, 10.0 g. 

 asparagin and added 0.2 to 1.0 g. 

 HBr. 



(g) Used 20.0 g. asparagin, 20.0 g. glycerol 

 and added 0.2 to 1.0 g. KI. 



(h) Used 10.0 g. asparagin, 20.0 g. 

 glj'cerol and made no additions, or 

 added 0.2 to 1.0 g. KCl, 0.2 to 1.0 g. 

 K2SO4, or 0.2 to 1.0 g. NaoS04. 

 Reference: Sullivan (1905-06 p. 127-140). 



389. Waksman's Basal Glycerol Asparagin 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol 30.0 g 



3. K2HPO4 1.0 g 



4. KCl 0.5 g 



5. MgS04 0.5 g 



6. FeS04 0.01 g 



7. Asparagin 5.0 g, 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Add 2.0 g. of one of the added nu- 

 trients. 



(3) Tube in 10-12 cc. lots. 



(4) Adjustment of reaction not specified. 

 Sterilization: Sterilize at 15 pounds for 



15 minutes. 

 Use: To study metabolism of Actinomy- 



cetes. 

 Added nutrients: The author added 2.0 g. 

 of one of the following: 



NaNOa (NH4)2S04 



NaXOa (XH 4)200 3 



Reference: Waksman (1920 p. 3). 



390. Tanner's Basal Lactate Asparagin 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. XaCl 5.0 g. 



3. CaH4(P04)2-H20 2.0 g. 



4. Ammonium lactate 6.0 g. 



5. XaOH (normal) 20.0 cc. 



6. Asparagin 4.0 g. 



