114 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add one of the listed added nutrients. 



(3) Tube. 



Sterilization: Sterilize in the Arnold 



Sterilizer. 

 Use: To study metabolism of sulphur. 



Production of H2S by the fluorescent 



group, colon-typhoid group and others. 



H2S production may be detected by the 



blackening of lead acetate paper. 

 Added nutrients: One of the following 



materials was employed: 



Cystine (prepared from wool, Folin's 

 method) 60.0 g. 



2-thiohydantoin 1.0 cc. to each 5.0 cc. of 

 medium. 



Thiourea 100.0 g. 



Taurine, amount not given. 



Na2S203-5H20 3.0 g. 



Na2S03 30.0 g. 



Reference: Tanner (1917 p. 586). 



391. Hiss' Basal Litmus Asparagin Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 2.0 g. 



3. MgS04 0-4 g- 



4. NaCl 5.0 g. 



5. Asparagin 4.0 g. 



6. Ammonium lactate 5.0 cc. 



7. Litmus (5.0% soln.) 15.0 cc. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add 6.0 cc. of normal NaOH. 



(3) Add 15.0 cc. of 5.0% litmus solution. 



(4) Add 1.0% of one of the carbon sources 

 given in added nutrients. 



(5) The medium is clear. 

 Sterilization: Method not given. . 



Use: To study fermentation by the dysen- 

 tery group. Acid production turns the 

 medium from purplish blue to red. 

 Added nutrients: The author added 1.0% 

 of one of the following: 



glucose de.xtrin 



maltose mannitol 



sucrose 

 Reference: Hiss (1904 p. 32). 



392. Harvey's Basal Lactate Asparagin 

 Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Ammonium lactate 5.0 g. 



3. Na2S04 2.0 g. 



4. MgS04 0.2 g. 



5. Asparagin 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: To ascertain production of indol test. 



Added nutrients: Author added 30.0 g. of 

 peptone, or 0.1, 0.3, or 0.5 g. of 1-1000 

 tryptophane. 



Variants: The basic solution was used as 

 culture medium without further addi- 

 tions. 



Reference: Harvey (1921-22 p. 102). 



393. Capaldi and Proskauer's Basal Citrate 

 Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Asparagin 2.0 g. 



3. MgS04 2.0 g. 



4. Citric acid 5.0 g. 



5. KH2PO4 2.0 g. 



6. CaCl2 0.2 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add NaOH until the reaction is 

 slightly alkaline. 



(3) Add 0.2% of one of the carbon sources 

 listed under added nutrients. 



Sterilization: Not specified. 

 Use: To study fermentation by members 

 of the colon-typhoid group. The authors 

 reported no growth of Bacterium hjphi. 

 B. colt produced gas from glucose, levu- 

 lose, lactose and mannose. 

 Added nutrients: 0.2% of one of the follow- 

 ing was added: 



glucose maltose 



levulose raffinose 



lactose maltose 



sucrose mannose 



Reference: Capaldi and Proskauer (1896 

 p. 454). 



394. Mendel's Basal Citrate Asparagin 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. KNO3 2.0 g. 



3. Asparagin 5.0 g. 



4. MgS04 2.0 g. 



5. Citric acid 5.0 g. 



6. K2HPO4 2.0 g. 



7. CaCl2 0.2 g. 



8. Iron chloride 



