116 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. Citric acid 0.75 g. 



4. Glycerin 10.0 g. 



5. Potassium biphosphate — 5.0 g. 



6. MgS04 2.5 g. 



7. (NHO2SO4 5-0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Adjustment of reaction not specified. 

 Sterilization: Sterilize for 30 minutes on 



each of two successive days. On the 

 third day add 1.0% of one of the added 

 nutrients and sterilize for 15 minutes. 



Use: To study decomposition of sugars by 

 Bacterium coli and Bacterium Fitzianus. 



Added nutrients: The author added 1.0% 

 of one of the following: 



glucose lactose 



maltose sucrose 



Reference: Mendel (1911 p. 297). 



398. Beijerinck and Minkman's Nitrate 

 GlycocoU Solution 



Constituents : 



1. Water 1000.0 cc. 



2. GlycocoU (2.5%) 25.0 g. 



3. NaNOs (1.0%) 10.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: To study denitrification by soil forms, 

 {Bacillus nitroxus). The medium was 

 inoculated with soil. 



Reference: Beijerinck and Minkman (1910 

 p. 37). 



399. Bokorny's Sucrose GlycocoU Solution 



Constituents: 



1. Water 1000.0 cc. 



2. GlycocoU 2.5 g. 



3. Sucrose 50.0 g. 



4. KH2PO4 2.0 g. 



5. MgS04 1-0 g- 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 SterUization: Not specified. 



Use: Cultivation of Yeast. 

 Reference: Bokorny (1921 p. 336). 



400. Hadley's Glycerol GlycocoU Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Ammonium lactate 7.5 g. 



3. FeP04 0.8 g. 



4. Glycerin 30.0 g. 



5. GlycocoU 10 g. 



6. MgS04 3.2 g. 



7. K2HPO4 2.3 g. 



8. NaCl 6.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 and 



8 in sufficient water to make 1000.0 cc. 

 SterUization: Not specified. 

 Use: Cultivation of Bacillus diphtheriae 



and study of toxin production. 

 Variants: The author omitted the FePO* 



and added 0.8 g. CaCU. 

 Reference: Hadley (1907 p. 101). 



401. Beijerinck and Minkman's Nitrate 

 Alanine Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Alanine (0.5%) 5.0 g. 



3. KNO3 (1.0%) 10.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Inoculate with soil. 

 SteriUzation: Not specified. 



Use: To study denitrification by soU forms, 



Bacillus nitroxus. 

 Reference: Beijerinck and Minkman (1910 



p. 37). 



402. Berthelot's Tyrosine Salt Solution 



Constituents : 



1. Water 1000.0 cc. 



2. K,S04 0.2 g. 



3. MgS04 0.2 g. 



4. K2HPO4 0.5 g. 



5. KNO3 0.25 g. 



6. CaCl2 0.02 g. 



7. Tyrosine 2.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 



Sterilization: Not specified. 



Use: Enrichment and isolation— BaciWus 

 phenologenes. Bacillus phenologenes is 

 enriched in this medium and then plated 

 on the same medium solidified with 



Berthelot (1913 p. 19). 



agar. 

 Reference: 



403. Vansteenberge's Glucose Tyrosine 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose 20.0 g. 



3. Tyrosine 0.2 g. 



4. K2HPO4 0.5 g. 



5. MgS04 2.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 



