CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



117 



sterilization: Not specified. 

 Use: Cultivation of B. coli. 

 Reference: Vansteenberge (1917 p. 609). 



404. Bokorny's Sucrose Tyrosine Solution 

 Constituents: 

 1- Water 1000.0 cc. 



2. Tyrosine 2.5 g. 



3. Sucrose 50 g 



4. KH2PO4 2 0g' 



5. MgS04 1.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not given. 



Use: Cultivation of yeast. 

 Reference: Bokorny (1917 p. 340). 



405. Waksman's Glycerol Tyrosine Solution 

 Constituents: 

 1- Water 1000.0 cc. 



2. K2HPO4 10 



3. MgS04 0.5 I. 



4. KCI 0.5 g. 



5. FeS04 0.01 g 



6. Tyrosine 1.0 g 



7. Glycerol 3O.0 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Tube in 10-12 cc. lots. 



(3) Adjustment of reaction not specified. 

 Sterilization: Sterilize at 15 pounds for 



15 minutes. 

 Use: To show utilization of tyrosine by 



Actlnomycetes. 

 Reference: Waksman (1920 p. 18). 



406. Myers' Cystine Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. Cystine 5.0 g. 



Preparation: (1) Dissolve the cystine in 1, 



using enough NajCOs to keep the cystine 



in solution. 

 Sterilization: Sterilize by filtration, then 



tube and test sterility. 

 Use: To study H,S production. If H2S is 



formed, lead acetate paper is blackened. 



No growth appeared with organisms 



studied unless medium was acid. Some 



organisms produce H2S in acid medium. 

 Variant: The author gives the following 



variant : 



(a) Add a bit of sterile litmus paper to 

 each tube and sterile 5.0% HCl until 

 the litmus is faintly red. The cys- 

 tin is precipitated at neutrality. 



(b) Inoculate and incubate. 



(c) After a few days litmus is turned blue 

 again and more HCl is added. 



(d) Suspend a sterile piece of lead acetate 

 soaked filter paper in each tube to 

 test or H2S production. 



Reference: Myers (1918 p. 250). 



407. Heap and Cadness' Cystine Solution 

 Constituents: 



1. Distilled water 1000 cc 



2. NaCl 5.0g.' 



3. Na2HP04 4.2 g. 



4. Sodium citrate 6.0 g. 



5. Cystine 0.2 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: To study hydrogen sulphide produc- 

 tion by B. aertnjcke. The authors re- 

 ported that the addition of glucose to 

 this medium gave earlier and accelerated 

 hydrogen sulphide production, but pro- 

 duction ceases after 24 hours. 



Reference: Heap and Cadness (1924-25 

 p. 86). 



408. Rogers, Clark and Evans' Tryptophane 



Solution 

 Constituents : 

 1- Water iqqo.O cc. 



2. Tryptophane 03g 



3. K2HPO4 5.0 g.' 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Tube in 10.0 cc. lots. 

 Sterilization: Not specified. 



Use: To study production of indol. If 

 indol is present a violet color appears 

 when 1.0 cc. of a 2.0% alcoholic solution 

 of p-dimethylamidobenzaldehyde and 

 concentrated HCl are added (Zipfel's 

 method). 



Variants: Braun and Cahn-Bronner used 

 0.5% NaCl, 0.4% tryptophane, 0.2% 

 K2HPO4 and added 0.7% normal NaHCO, 

 after the solution was neutralized to 

 litmus. 



References: Rogers, Clark and Evans 

 (1914 p. 101), Braun and Cahn-Bronner 

 (1921 p. 199), Harvey (1921-22 p. 117). 



409. Frieber's Glucose Tryptophane 

 Solution 

 Constituents : 



1- Water looO.O cc. 



2. Tryptophane (0.03%) 0.3 g. 



