118 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. NaCl(0.5%) 5.0 g. 



4. Potassium phosphate 



(0.2%) 2.0 g. 



5. MgSO4(0.02%) 0.2 g. 



6. Glucose (3.0%) 30.0 g. 



7. CaCOa 

 Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Adjustment of reaction not given. 



(3) Distribute in flasks in 50.0 cc. lots. 



(4) Add 10.0%, sterile CaCOa to each 

 flask. 



Sterilization: Not specified. 



Use: To study indol production. The 

 author reported that indol was not 

 formed in the presence of glucose. 



Reference: Frieber (1921-22 p. 268). 



410, Bokorny's Sucrose Leucine Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Leucine 2.5 g. 



3. Sucrose 50.0 g. 



4. KH2PO4 2.0 g. 



5. MgS04 1-0 g- 



Preparation: (1) Dissolve one of 2, 3, 4 



and 5 in 1. 

 Sterilization: Not specified. 

 Use: To study the nutrient requirements 



for growth of yeast. 

 Reference: Bokorny (1917 p. 340). 



411. Dolt's Ammonium Asparagin Solution 

 No. I 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Asparagin 10.0 g. 



3. (NH4)2HP04 2.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Make neutral to phenolphthalein 

 with NaOH. 



Sterilization: Not specified. 



Use: Cultivation of colon group and organ- 

 isms found in water. 



Reference: Dolt (1908 p. 620), Tanner 

 (1919 p. 65). 



412. Kendall, Walker and Day's Ammo- 

 nium Asparagin Solution 



Constituents : 



1. Redistilled water 1000.0 cc. 



2. Asparagin 2.0 g. 



3. (NH4)2HP04 2.0 g. 



4. Na2HP04 10 g- 



5. NaCl 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Adjustment of reaction not given. 



(3) Distribute in 100.0 cc. lots. 

 Sterilization: Method not given. 



Use: To study lipolytic and proteolytic 

 activity by the tubercle bacilli. To 

 determine lipolytic action, suspend 1.0 

 cc. of the bacteria free filtrate of the 

 culture in freshly boiled distilled water. 

 Add 0.25 cc. of ethylbutyrate and 0.5 

 cc. of toluene and incubate at 37°C. The 

 increase in acidity as measured in terms of 

 N/50 NaOH measures lipolytic activity. 



Variants : The authors used 4.0 g. asparagin 

 and omitted the Na2HP04. 



Reference: Kendall, Walker and Day (1914 

 p. 463). 

 413. Dolt's Asparagin Solution No. 2 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Asparagin 10-0 §• 



3. Na2HP04 2.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Neutralize with NaOH using phenol- 

 phthalein as an indicator. 



Sterilization: Method not given. 



Use: Cultivation of colon group and organ- 

 isms found in water. 



Variants: Dolt added 5.0 g. NaCl and 

 neutralized the medium by the addition 

 of Na.CO.s 



References: Dolt (1908 pp. 617, 620), 

 Tanner (1919 p. 65). 



414. Beijerinck and Minkman's Nitrate 

 Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Asparagin (0.05%) 0.5 g. 



3. KNO3 (1.0%) 10.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Inoculate with soil. 

 Sterilization: Not specified. 



Use: To study denitrification by soil forms, 

 Bacillus pyocyaneus. 



Variants: Author employed the above me- 

 dium with the addition of 5.0 g. K2HPO4. 



Reference: Beijerinck and Minkman (1910 

 p. 37). 



