CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



119 



415. Blanchetiere's Asparagin Salt Solution 



Constituents: 



1. Distilled water 900.0 cc. 



2. NaCl 5.0 g. 



3. Na2HP04 1.0 g. 



4. Potassium biphosphate 1-0 g. 



5. Asparagin 3.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in enough water 

 to make 900.0 cc. 



(2) Distribute in 450.0 cc. lots in liter 

 flasks. 



Sterilization: Sterilize for 15 minutes at 



110° to 112°C. 

 Use: To study asparagin decomposition by 



Bacillus fluorescens liquefaciens (Flugge). 

 Reference: Blanchetiere (1917 p. 294). 



416. Park, Williams and Krumwiede's 



Asparagin Salt Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Asparagin 6.0 g. 



3. Sodium phosphate (ortho).. 2.0 g. 



4. NaCl 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) If necessary make alkaline to litmus 

 by the addition of NaOH. 



(3) Tube in small quantities. 



(4) Test for color production with. B. 

 pj/ocyaneus . 



Sterilization: Method not given. 

 Use : Culture medium for disinfection tests. 

 Reference: Park, Williams and Krum- 

 wiede (1924 p. 122). 



417. Bokorny's Asparagin Salt Solution 

 Constituents : 



1- Water 1000.0 cc. 



2. Asparagin (0.5%) 5.0 g. 



3. KH2PO4 (0.1%) 1.0 g. 



4. MgS04 (0.03%) 0.3 g. 



5. CaCIo trace 



6. K2SO4 (0.1%) 1.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization: Not specified. 



Use: To study effect of metallic salts on 

 yeast growth. The author reported that 

 4.0% of K2SO4 did not inhibit the develop- 

 ment of the yeasts. 



Variants: The author used 0.5, 1.0, 2 or 

 4.0% K2SO4. 



Reference: Bokorny (1912 p. 122), 



418. Uschinsky's Asparagin Salt Solution 



(Giltner) 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. Asparagin 34g 



3. NaCl 5.0 g. 



4. MgS04 0.2 g. 



5. CaCh 0.1 g. 



6. KH2PO4 1.0 g. 



7. FeS04 trace 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Variants : 



(a) Gottheil did not specify the type of 

 phosphate used and used 0.3 g. MgS04 

 with 10.0 g. asparagin. 



(b) Linde used 0.01 g. Fe2Cl6 instead 

 of FeS04, used 0.3 g. MgS04-7H20, 

 0.1 g. NaCI, and did not specify the 

 amount of asparagin added. H used 

 the solution with an alkaline reaction 

 for the cultivation of Cladothrix. 

 The addition of glucose, sucrose or 

 glycerol would have increased the 

 amount of growth. 



References: Giltner (1921 p. 369), Gottheil 

 (1901 p. 432), Linde (1913 p. 386). 



419. Henneberg's Asparagin Salt Solution 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. Asparagin (0.075 to 



0.9%) 0.75 to 9.0 g. 



3. K2HPO4 (0.05 to 



1-5%) 0.5 to 15.0 g. 



4. MgS04 (0.02 to 



0.6%) 0.2 to 6.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of yeast. 

 Variants : 



(a) Bokorny used 25.0 g. asparagin, 5.0 g. 

 KH2PO4 and 1.0 g. MgS04. 



(b) Evans used 5.0 g. asparagin, 1.0 g. 

 MgS04 and 2.0 g. K2HPO4. 



(c) Heinemann and Tanner used 2.0 g. 

 asparagin, 1.0 g. MgS04 and 1.0 g. 

 K2HPO4. 



(d) Tanner used 10.0 g. asparagin, 2.0 g. 

 MgS04 and 1.0 g. KH2PO4 for pig- 

 ment production by Ps. pyoctjaneus 

 and Ps. fluorescens liquefaciens. 



(e) Boehncke used 2.0% asparagin, 0.5% 



