120 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



NaCl and 0.05% MgS04 to determine 

 ammonia production by Proteus. 

 References: Henneberg (1907 pp. 40-45), 

 (1909 p. 104), Bokorny (1917 p. 338), 

 Evans (1916 p. 444), Heinemann (1922 

 p. 42), Tanner (1919 pp. 64, 66), Boehncke 

 (1911 p. 74). 



420. Amaud and Charrin's Asparagin Salt 



Solution (Roux and Rochaix) 



Constituents: 



1. Water 1000.0 cc. 



2. KH2PO4 01 g- 



3. Na2HP04-12H20 0.1 g. 



4. KHCO3 0.134 g. 



5. CaCU 0.05 g. 



6. MgS04-7H20 0.05 g. 



7. Asparagin 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation oi Bacillus pyocyaneus. 

 Reference: Roux and Rochaix (1911 p. 104). 



421. Saltet's Glucose Asparagin Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. NaCl 12.0 g. 



3. KCl 10 g. 



4. Na2HP04 0.40mg. 



5. Glucose 0.5 g. 



6. Asparagin 1-0 g- 



7. Na2S04 varying amounts 



Preparation: 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add varying amounts of Na2S04 

 (amount not given) to (1). 



Sterilization: Not specified. 



Use: To study sulphate reduction by B. 



desulfuricans . Sulphates were reduced. 

 Variants: The author substituted MgS04 



for Na2S04. 

 Reference: Saltet (1900 p. 699). 



422. Saltet's Thiosulphate Asparagin 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NaCl 12.0 g. 



3. KCl 10 g. 



4. Na2HP04 30.0 mg. 



5. Na2S04 1-0 g- 



6. Na2S203 1-5 g- 



7. Asparagin 1-0 g. 



8. Glucose 1-5 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 

 and 8 in 1. 



Sterilization: Not specified. 



Use: To study thiosulphate reduction by 

 B. desulfuricans and B. coli. When 

 ammonia and sodium nitroprusside were 

 added to either culture a negative reac- 

 tion was obtained. 



Reference: Saltet (1900 p. 702). 



423. Blanchetiere's Glucose Asparagin 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NaCl 5.0 g. 



3. Na2HP04 10 g. 



4. K2HPO4 10 g. 



5. Asparagin 5.0 g. 



6. Glucose 3.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in enough 

 water to make 1000.0 cc. 



(2) The reaction is alkaline to litmus 

 before sterilization and neutral after 

 sterilization. 



Sterilization: Autoclave at 112° for 40 



minutes. 

 Use: To study asparagin decomposition by 



Bacillus fluorescens liquefaciens (Flugge). 



Good growth when NH4CI is added but 



no pigment is produced. 

 Variants: The author specified that 5.0 g. 



NH4CI might be added. 

 Reference: Blanchetiere (1917 p. 301). 



424. Capaldi and Proskauer's Glucose 

 Asparagin Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Asparagin (0.2%) 2.0 g. 



3. NaCl (0.02%) 0.2 g. 



4. MgS04 (0.01%) 0.1 g. 



5. CaCl2 (0.02%) 0.2 g. 



6. KH2PO4(0.2%) 2.0 g. 



7. Dextrose (0.2%) 2.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 



Sterilization: Not specified. 



Use: Acid production by Bacterium coli 

 and typhoid bacillus. 



Variants: Robertson and Davis used the 

 following solution to study the influence 

 of vitamines on bacterial growth. All 

 materials were chemically pure. This 



