CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



121 



medium did not give continued growth 

 of yeast. 



1. Sterile distilled water 1000.0 cc. 



2. Asparagin (Merck) 3.4 g. 



3. CaCl2 0.1 g. 



4. Dextrose 20.0 g. 



5. MgS04 0.2 g. 



6. K2HPO4 1.0 g. 



7. NaCl 5.0 g. 



References: Capaldi and Proskauer (1896 



p. 456), Robertson and Davis (1923 p. 154). 



425. Gottheil's Glucose Asparagin Solution 

 No. X 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 10 g. 



3. CaCla 1.0 g. 



4. MgS04 0.3 g. 



5. NaCl 0.1 g. 



6. Iron trace 



7. Asparagin 10.0 g. 



8. Glucose 30.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 and 



8 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of organisms found in the 



soil, on roots and on rhizomes. 

 Reference: Gottheil (1910 p. 432). 



426. Capaldi and Proskauer's Glucose 

 Asparagin Solution No. II 



Constituents : 



1. Water 1000.0 g. 



2. Asparagin 2.0 g. 



3. Glucose 2.0 g. 



4. NaCl 5.0 g. 



5. MgS04 2.0 g. 



6. Sodium phosphate 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add NaOH until the reaction is 

 slightly alkaline. 



Sterilization: Not specified. 



Use: Acid production by Bacteriuyn coli 



and typhoid bacilli. 

 Reference: Capaldi and Proskauer (1896 



p. 456). 



427. Stutzer's Glucose Asparagin Solution 



Constituents: 



1. Water 1000.0 g. 



2. Glucose 10.0 g. 



3. Asparagin 20 g 



4. KH2PO4 0.5 g. 



5. MgS04 0.25 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of bacteroids from Pisum 



sativum. 

 Variants : 



(a) Hefferan used 1.0 g. MgS04, 1.0 g. 

 sodium phosphate and 20.0 g. of 

 glucose to cultivate B. rosaceus 

 metalloides. This organism forms 

 rosette-like groupings in liquid media. 

 The addition of glucose aids the 

 growth of the organism. 



(b) Kuntze used the following solution 

 to study pigment production by 

 Bacillus ruber indicus. He reported 

 that the organism produced pigment 

 when grown in medium without 

 MgS04 for 6 days and then in medium 

 containing MgS04. 



1. Distilled water 1000.0 cc. 



2. d-glucose (Soxhlet) . 25.0 g. 



3. Asparagin 10.0 g. 



4. K2HPO4 2.0 g. 



5. MgS04 0.0 or 2.0 g. 



(c) Kuntze used the following solution 

 to study pigment production by 

 Aspergillus niger and Bacillus prodi- 

 giosus. 



1. Distilled water 1000.0 cc. 



2. MgS04 1.0 to 2.0 g. 



3. K2HPO4 2.0 g. 



4. d-glucose 25.0 g. 



5. Asparagin 10.0 g. 



(d) Kuntze used the following solution to 

 study pigment production by Bacil- 

 lus prodigiosus. He reported that 

 when KH2PO4 was used pigment was 

 produced: 



1. Distilled water 1000.0 cc. 



2. Asparagin 10.0 g. 



3. d-glucose 20.0 g. 



4. KH2PO4 or K2HPO4 2.0 g. 



5. MgS04 2.0 g. 



(e) Zikes used a solution containing 

 100.0 g. glucose, 2.5 g. asparagin and 

 3.3 g. MgS04 per liter to determine 

 volutin production by Oidiurn lactis. 

 He distributed the solution in 10.0 cc. 

 quantities and added 0.25, 0.125, 

 0.063, 0.032 or 0.016 g. K2HPO4 to 

 each tube. He reported that de- 



