CULTUEE MEDIA FOR CULTIVATION OF MICROORGANISMS 



123 



(6) Dry at 60°C. until all traces of damp- 

 ness of alcohol or ether have dis- 

 appeared. 



(7) Prepare a 10.0% solution of (6) in 

 distilled water. 



(8) Mix about 10.0% of (7) with 10.0% 

 of (1). 



(9) Distribute in 150.0 cc. lots in fer- 

 mentation flasks. 



Sterilization: Sterilize in streaming steam. 

 Use: To study fermentation by yeast. 

 Reference: Korff (1898 p. 532). 



434. Zikes' Magnesium Sucrose Asparagin 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Asparagin 10.0 g. 



3. K2SO4 5.0 g. 



4. MgS04 2.5 g. 



5. Saccharose 75.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: To study perithecium formation by 



Aspergillus oryzae. 

 Reference: Zikes (1922 p. 342). 



435. Chrzaszcz's Sucrose Asparagin 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Sucrose 100.0 g. 



3. Mg(H2P04)2 2.0 g. 



4. Ca(H2P04)2 2.0 g. 



5. K2SO4 2.0 g. 



6. Asparagin 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization: Not specified. 



Use: To study growth requirements for 

 yeasts. Author reported that yeast de- 

 veloped very well in this medium. 



Reference: Chrzaszcz (1904 p. 149). 



436. Sullivan's Glycerol Asparagin 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Asparagin 10.0 g. 



3. K2HPO4 1.0 g. 



4. MgS04 0.2 g. 



5. Glycerol 20.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of chromogenic bacteria. 

 The author reported that the chromogenic 

 bacteria developed rapidly with good 

 color production on this medium. 



Variants: (1) Bezangon used 2.5 g. MgS04, 

 1.5 g. potassium phosphate, 4.0 g. aspara- 

 gin and 15.0 g. glycerol. This solution 

 was used for the cultivation of tubercle 

 bacilli. 



Reference: Sullivan (1905-06 p. 116), 

 Bezangon (1920 p. 547). 



437. Schweinitz and Dorset's Glycerol 



Asparagin Solution (Goris) 

 Constituents : 



1. Water 1000.0 cc. 



2. Glycerol 70.0 g. 



3. KH2PO4 1.0 g. 



4. Ammonium phosphate 10.0 g. 



5. NaCl 10.0 g. 



6. Asparagin 2.0 g. 



7. MgS04 2.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of tubercle bacilli. 

 Reference: Goris (1920 p. 499). 



438. Waksman and Joflfe's Glycerol Aspara- 



gin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol 30.0 g. 



3. MgS04 0.5 g. 



4. KCl 0.5 g. 



5. FeS04 0.01 g. 



6. Asparagin 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Adjust medium to different pH from 

 4.4 to 8.7 with phosphates and 

 carbonates. 



(3) Tube in 10-12 cc. lots. 

 Sterilization: Sterilize at 15 pounds for 



15 minutes. 

 Use: To study effect of reaction (pH) on 



metabolism of actinomycetes. 

 Reference: Waksman and Joffe (1923 p. 44). 



439. Hadley's Glycerol Asparagin Solution 



Nos. Ill and IV 



Constituents : 



1. Water 1000.0 cc. 



2. Ammonium phosphate 10.0 g. 



