124 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. Asparagin 10.0 g. 



4. CaCl2 0.1 g. 



5. Glycerin 40.0 g. 



6. KNO3 0.3 g. 



7. K2HPO4 0.3 g. 



8. NaCl 6.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 



and 8 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of Bacillus diphtheriae 



and study of toxin production. 

 Variants: The author used 0.3 g. MgS04 



instead of 0.6 g. NaCl for solution No. 4. 

 Reference: Hadley (1907 p. 101). 



440. Peklo's Mannitol Asparagin Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. KH2PO4 15.0 g. 



3. CaCU 10 g. 



4. MgS04 3.0 g. 



5. NaCl 1.0 g. 



6. FeiCle (Merck siccum) 



7. Mannite 20.0 g. 



8. Asparagin 4.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 (amount of 6 

 not given) in 1. 



(2) Neutralize with K2CO3. 



(3) Partially correct the precipitate loss 

 by the addition of orthophosphoric 

 acid. 



(4) Dissolve 7 and 8 in (3). 



(5) Reaction is found to be neutral after 

 sterilization. 



Sterilization: Method not given. 



Use: Cultivation of plant actinomyces. 



Variants : 



(a) Peklo prepared the following solu- 

 tion: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 5.0 g. 



3. KH2PO4 4.5 g. 



4. MgS04 3.0 g. 



5. NaCl 10 g. 



6. CaCh 1-0 g. 



7. FesCle trace 



He diluted 200.0 cc. of this solution 

 with 800.0 cc. of distilled water and 

 added 20.0 g. mannitol and 4.0 g. 

 asparagin. This solution was steril- 

 ized twice for one hour. The final 

 reaction is weakly acid. 



(b) Peklo added 20.0 g. of mannitol, 4.0 g. 

 asparagin and 100.0 cc. of Meyers 

 solution (preparation not given) to 

 900.0 cc. of distilled water. The mix- 

 ture was boiled 30 minutes. Add 

 soda until the reaction is strongly 

 alkaline. 

 Reference: Peklo (1910 pp. 509, 510, 522). 



441. Capaldi and Proskauer's Mannitol 

 Asparagin Solution I 



Constituents: 



1. Distilled water 



2. Asparagin (0.2%) 



3. Mannite (0.2%) 



4. NaCl (0.02%) 



5. MgS04 (0.01%) 



6. CaCla (0.02%) 



7. KH2PO4 (0.2%) 



8. Litmus. 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Steam for 30 minutes in the steamer. 



(3) Add NaOH to obtain a neutral reac- 

 tion to litmus. 



(4) Add litmus to color. 



(5) Sterilize again for 30 minutes in the 

 steamer. 



(6) Filter. 



(7) Tube in 5.0 cc. lots. 



(8) Plug the tubes with cotton. 

 Sterilization: Final sterilization not speci- 

 fied. 



Use : Differentiation of colon-typhoid group 

 of bacteria. B. coli produced acid, 

 typhoid bacillus produced no change. 



Variants: Harvey used 2.0 g. NaCl. 



Reference: Capaldi and Proskauer (1896 

 p. 472), Harvey (1921-22 p. 102). 



441a. Ellrodt's Acetate Asparagin Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Asparagin 5.0 g. 



3. Sodium acetate 5.0 g. 



4. Potassium phosphate 2.0 g. 



5. NaCl 2.0 g. 



6. MgS04 0.1 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



inl. 

 Sterilization: Not given. 

 Use: Cultivation of Baci. pyocyaneuin. 

 Reference: Ellrodt (1902 p. 641). 



