CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



125 



441b. Voges' Lactate Asparaginate Solution 



Constituents : 



1. Distilled water 200.0 cc. 



2. NaCl 8.0 g. 



3. K2HPO4 2.0 g. 



4. Ammonium lactate 6.0 g. 



5. Sodium asparaginate 4.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Method not given. 

 Use: Enrichment and isolation of cholera 



bacilli from water. Add 800.0 cc. of the 



water to be investigated to the medium. 



Used also as a general culture medium. 

 Variants : 



(a) Sames used 5.0 g. NaCl, 4.0 g. aspara- 

 gin and 8.0 g. ammonium lactate. 

 He distributed the solution in 5.0 cc. 

 lots and added 0.0, 0.2, 0.25, 0.3, 

 0.35, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 or 1.1 

 cc. of a 5.0% NaOH solution to each 

 tube. He reported that motile sar- 

 cine grew best when 0.25 to 0.3 cc. of 

 5.0% NaOH solution was added to 

 each 5.0 cc. medium. 



(b) Heinemann used 2.0 g. Na2HP04 

 instead of K2HPO4, 5.0 g. NaCl, 

 6.3 g. ammonium lactate and 3.4 g. 

 asparagin. 



(c) Kuntze used 2.0 g. KH0PO4 instead 

 of K2HPO4, 4.0 g. asparagin and 5.0 g. 

 NaCl. He used this medium to study 

 pigment production by Bacillus pro- 

 digiosus. 



(d) Dolt used 5.0 g. NaCl, 2.0 g. Na2HP04 

 instead of K2HPO4 and 4.0 g. aspara- 

 gin. The solution was neutralized 

 by the addition of Na2C03. 



(e) NicoUe, Raphael and Debaiens used 

 5.0 g. NaCl and 4.0 g. of asparagin. 



(f) Magnusson used 5.0 g. NaCl. The 

 medium was used to study slime pro- 

 duction by Bacterium lactic viscosum 

 and streptococci. 



(g) Tanner used 5.0 g. NaCl. 



(h) Bezangon used from 5.0 to 7.0 g. 

 NaCl, 6.0 to 7.0 g. ammonium lactate 

 and 3.0 to 4.0 g. asparagin. 



(i) Abbott, Dopter and Sacquepee, and 

 also Park, Williams and Krumuriede 

 used 4.0 g. asparagin, 5.0 g. NaCl, 

 and 2.0 g. Na2HP04. Abbott culti- 

 vated Pseudomonas aeruginosa, the 

 bacillus of green pus, on this medium. 



References: Voges (1894 p. 453), Sames 

 (1898 p. 665), Heinemann (1905 p. 131), 

 Kuntze (1907 p. 300), Dolt (1908 p. 617), 

 Nicolle, Raphael and Debaiens (1917 

 p. 378), Magnusson (1918 p. 467), Tanner 

 (1919 p. 68), Bezangon (1920 p. 116), 

 Abbott (1921 p. 385), Dopter and Sac- 

 quepee (1921 p. 122), Park, Williams and 

 Krumwiede (1924 p. 122). 



441c. Fraenkel and Voges' Salt Asparagin 

 Solution (Besson) 



Constituents: 



1. Water 



2. NaCl 



3. CaCl2 



4. MgS04 



5. K2HPO4 



6. Ammonium lactate 



7. Asparagin 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1 



(2) Neutralize. 

 Sterilization: Method not given. 

 Use: General culture medium. 

 Reference: Besson (1920 p. 37). 



442. Frankel's Lactate Asparagin Solution 

 (Tanner) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. NaCl 5.0 g. 



3. CaHP04 2.0 g. 



4. Ammonium lactate 6.0 g. 



5. Asparagin 4.0 g. 



6. NaOH (normal) 20.0 cc. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Filter. 



Sterilization: Sterilize in Arnold on 3 suc- 

 cessive days. 



Use: For cultivation of green fluorescent 

 bacteria. This medium aided in pigment 

 production of strains studied. 



Variants : 



(a) Tanner used 2.0 g. Ca(HP04)2 in- 

 stead of CaHPO*. 



(b) Lohnis used 2.0 g. of K2HPO4, 

 Ca(HP04)2 and made distinctly alka- 

 line by the addition of NaOH, instead 

 of adding 20.0 cc. of normal NaOH. 



References : Tanner (1918 p. 82), (1919 p. 64), 

 Lohnis (1913 p. 44), Klimmer (1923 p. 173), 



