126 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



443. Uschinsky's Lactate Asparaginate 



Solution (Smith) 



Constituents : 



1. Distilled water 1000.0 ce. 



2. Ammonium lactate 5.0 g. 



3. Sodium asparaginate 2.5 g. 



4. Na2SO4-10H2O 2.5 g. 



5. NaCl 2.5 g. 



6. K2HPO4 2.5 g. 



7. CaClj 0.01 g. 



8. MgS04 0.01 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 and 



Sin 1. 

 Sterilization: Not specified. 

 Use: As a general culture medium. 

 References: Smith (1905 p. 197), Tanner 



(1919 p. 69). 



444. Melick's Lactate Asparagin Solu-tion 



Constituents: 



1. Redistilled water 1000.0 cc. 



2. NaCl 5.0 g. 



3. Asparagin 3.4 g. 



4. Na2HP04 2.0 g. 



5. MgS04 0.5 g. 



6. Ammonium lactate 6.3 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Adjust to +0.2 acid by the addition of 

 NaaCOs. 



Sterilization : 



(1) Autoclave for 10 minutes at 10 pounds 

 pressure. 



(2) Tube and autoclave again at 10 

 pounds pressure for 10 minutes. 

 Discard all tubes showing a pre- 

 cipitate. 



Use: Growth of organisms for preparation 

 of bacterial antigens, in colon-typhoid 

 group. 



Reference: Melick (1922 p. 408). 



445. van Delden's Lactate Ammonia 

 Asparagin Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NaCl 30.0 g. 



3. K2HPO4 0.5 g. 



4. MgS04-7H20 2.5 g. (or more) 



5. Sodium lactate ... 5.0 to 10.0 g. 



6. Asparagin 10 g. 



7. Mohr's salt trace 



(FeS04(NH4)2S04). 



8. Na2S03 small quantity 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Inoculate with sea water or sand 

 from a beach. 



Sterilization: Not specified. 



Use: Enrichment of sulphite and sulphate 



reducers from sea water, Microspira 



aestuarii. 

 Variants: Omit the NaoSOs if inoculation 



is made with sea mud colored black with 



H2S. 

 Reference: van Delden (1903-04 p. 93). 



446. Beijerinck's Lactate Asparagin 

 Solution 



Constituents : 



1. Water (ditch) 1000.0 cc. 



2. Asparagin 2.5 g. 



3. MgS04 2.5 g. 



4. Potassium phosphate 2.0 g. 



5. Iron lactate 1 -0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in ditch water 

 ("Graben wasser"). 



(2) Make alkaline with NaaCOa. 

 Sterilization: Method not given. 



Use: To study the reduction of sulphates. 

 Author reported strong US and ammo- 

 nium sulfide production after 48 hours 

 at 30°C. 



Reference: Beijerinck (1895 p. 107). 



447. van Delden's Lactate Asparagin 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. Sodium lactate 5.0 g. 



4. Asparagin 10 g. 



5. MgS04-7H20 1.0 g. 



6. FeS04 trace 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not given. 

 Use: Sulphate reduction by Spirillum 



(Microspira) desuljuricans . Medium 



turned dark due to H2S production. 



H2S may also be determined by titration 



with 0.01 N iodine solution. 

 Variants : 



(a) The author substituted 1.0 g. 'gypsum 

 for MgS04. 



(b) Tanner and Giltner specified the use 

 of tap water. 



