CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



127 



References: van Delden (1903-04 p. S3), 

 Tanner (1919 p. 64), Giltner (1921 p. 377). 



448. Schroeder and Junger's Lactate 



Asparagin Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Asparagin 4.0 g. 



3. Sodium lactate 6.0 g. 



4. Sodium phosphate (Ortho) . 2.0 g. 



5. NaCl 5.0 g. 



Preparation : 



(1) Powder 2, 3, 4 and 5 separately. 



(2) Dissolve the powdered salts sepa- 

 rately in 50 to 100.0 cc. of water. 



(3) Mix the solutions and add the re- 

 mainder of the liter of water. 



(4) Add NaOH to make slightly alkaline 

 to litmus. 



(5) Filter. 



(6) Tube. 



Sterilization: Sterilize on 2 consecutive 

 days in the Arnold sterilizer. 



Use: Cultivation of Bacillus pyogenes and 

 chromogenes. The author reported that 

 a green band of pigment was produced 

 after 24 hours. The band gradually in- 

 creased until the whole tube was light 

 green. Non chromogenic organisms were 

 inhibited. 



Variants: Harvey used 2.0 g. of Na2HP04. 



References: Schroeder and Junger (1912 

 p. 601), Harvey (1921-22 p. 102). 



449. Remy's Organic Acid Asparagin 



Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Asparagin 6.0 g. 



3. Oxalic acid 0.5 g. 



4. Lactic acid 0.15 g. 



5. Citric acid 0.15 g. 



6. Na^HPO^ 5.0 g. 



7. MgS04 2.5 g. 



8. K2SO4 1.25 g. 



9. NaCl 2.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 



8 and 9 in 1. 

 Sterilization: Not specified. 

 Use: Isolation of typhoid bacilli. 

 Reference: Thoinot and Masselin (1902 



p. 337). 



450. Kuntze's Citrate Asparagin Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. KNO3 2.0 g. 



3. Asparagin 1-0 g. 



4. MgS04 2.0 g. 



5. Citric acid 5.0 g. 



6. KH2PO4 2.0 g. 



7. CaCl2 0.2 g. 



8. Iron chloride several drops 



Preparation : 



(1) Dissolve 2 and 3 in part of 1. 



(2) Dissolve 4, 5, 6, 7 and 8 in the re- 

 mainder of 1. 



(3) Neutralize (2) with KOH during the 

 heating. 



(4) Mix (3) and (1). 



(5) Distribute into 100.0 cc. lots in com- 

 bustion flasks. 



Sterilization: Method not given. 



use: To study morphology and physiology 

 of denitrifying organisms Bacillus deni- 

 trificans agilis (Ampola and Garino) and 

 Bacillus oxalaticus (Zopf). 



Variants : 



(a) Wojtkiewicz used 3.0 g. KNO3, 7.6 

 potassium citrate instead of citric 

 acid, used 0.2 g. CaCla and specified 

 a trace of FeClo. 



(b) Arnd and Harvey specified a trace of 

 FeCl2. 



References: Kuntze (1904 p. 3), Smith 

 (1905 p. 198), Wojtkiewicz (1914 p. 258), 

 Arnd (1916 p. 567), Harvey (1921-22 

 p. 102). 



451. Giltay and Aberson's Citrate 

 Asparagin Solution (Tanner) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. KNO3 2.0 g 



3. MgS04 2.0 g 



4. Citric acid 5.0 g 



5. K2HPO4 2.0 g 



6. CaCl2 0.2 g 



7. NaaCOs 4.25 g 



8. Asparagin 1.0 g 



Preparation : 



(1) Dissolve 2 and 8 in a little distilled 

 water. 



(2) Dissolved 3, 4, 5, 6 and 7 in distilled 

 water. 



