128 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(3) Mix (1) and (2) and make up to a 

 liter. 



(4) Tube in fermentation tubes. 



(5) Adjustment of reaction not specified. 

 Sterilization: Method not given. 



Use: Cultivation of denitrifying bacteria. 



Variants: Lohnis used 2.0 g. KH2PO4 in- 

 stead of K2HPO4, omitted the NajCOa 

 and added a trace of Fe2Cl6. 



References: Tanner (1919 p. 67), Lohnis 

 (1913 p. 97). 



452. Maassen's Basal Citrate Asparagin 



Solution (Lohnis) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Citric acid 7.0 g. 



3. Asparagin 10.0 g. 



4. K2HPO4 2.0 g. 



5. NasCOs (crystalline) 2.5 g. 



6. MgS04 0.4 g. 



7. CaCU 0.01 g. 



Preparation : 



(1) Neutralize 7.0 g. citric acid with pure 

 KOH. 



(2) Dissolve (1), 3, 4, 5, 6 and 7 in 1. 



(3) Add from 1.5 to 4.0% of one of the 

 nutrients listed below to (2). 



Sterilization: Sterilize on each of 3 suc- 

 cessive days. 



Use: As a general synthetic medium. 



Added nutrients: Lohnis added from 1.5 

 to 4.0% of one of the following materials: 

 sugars 

 alcohols 

 other sources of carbon 



Reference: Lohnis (1913 p. 44). 



453. Maassen's Malate Asparagin Solution 



Constituents : 



1. Distilled water 



2. Malic acid 



3. KOH 



4. Asparagin 



5. MgS04 



6. Na2HP04 



7. Soda 



8. CaCla 



Preparation : 



(1) Dissolve 7.0 g. malic acid in about 

 100.0 cc. distilled water. 



(2) Neutralize (1) with KOH. 



(3) Make volume to 1000.0 cc. 



(4) Dissolve 10.0 g. finely powdered 

 asparagin, 0.4 g. MgS04, 2.0 g. 

 Na2HP04 and 2.5 g. crystalline pure 

 soda. 



Sterilization: Method not given. 



Use: Cultivation of cholera vibrio. 



Variants: The malic acid may be replaced 

 by 1/10 equivalent amount of any utiliza- 

 ble organic acid. Sodium may replace 

 potassium. The ammonia salt of any 

 inorganic or organic acid may replace the 

 asparagin or the asparagin may be 

 replaced by different nitrogenous organic 

 compounds such as amides, amino acids, 

 urea, or creatinin. The amount of soda 

 added may be changed and the amount 

 of water added may be increased. The 

 addition of cane sugar, lactose, maltose, 

 galactose, dextrose or dextrin gives a 

 more luxuriant growth. 



References : Massen (1894 pp. 401, 404), also 

 abstract by Busse (1894 p. 923). 



454. Beijerinck's Malate Asparagin Solution 



Constituents: 



1. Water (ditch) 



2. Mohr salt (NH4)2S04 



3. MgS04 



4. NaCl 



5. Sodium malat 



6. Asparagin 



7. Potassium phosphate. 



8. Na2C03 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1 liter 

 of ditch water ("Grabenwasser") 

 containing 37.5 MgS04 per liter. 



(2) Distribute into flasks or beaker. 

 Cover the beaker with a glass plate 

 or fill the flask full to reduce the sur- 

 face exposed to the air. 



Sterilization: Not specified. 

 Use: To study reduction of sulphates. 

 The author reported the presence of H2S 

 and SO3 after 3 days. 

 Variants : 



(a) The author used the following solu- 

 tion to study sulphate reduction by 

 Spirillum tenue. The water used was 

 "Grabenwasser" and the medium 

 was made alkaline by the addition of 

 NajCOs 

 1. Water (ditch) 1000.0 cc, 



