CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



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2. Sodium malat (natrium 



malate) 10.0 g. 



3. Asparagin 5.0 g. 



4. Potassium phosphate 2.5 g. 



5. NaCl 2.5 g. 



6. Mohr salt (NH4)2S04 1.0 g. 



(b) Beijerinck detected the production of 



US and SO3 after 6 days cultivating 

 Spirillum destilfuricans in the follow- 

 ing solution: 



1. Water (ditch) 1000.0 cc. 



2. Gypsum (CaS04) 2.0 g. 



3. Sodium malat (natrium 



malate) 0.5 g. 



4. Asparagin 0.5 g. 



5. Potassium phosphate 0.1 g. 



6. Na.COs 



Reference: Beijerinck (1895 pp. 55, 56, 

 107). 



455. Zikes' Alcohol Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose (2.0%) 20.0 g. 



3. Asparagin (0.25%) 2.5 g. 



4. K2HPO4 (0.1%) 1.0 g. 



5. MgS04 (0.05%) 0.5 g. 



6. Alcohol 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Distribute in 10.0 cc. lots. 



(3) To each 10.0 cc. lot add 5, 10, 15 or 

 20 drops of alcohol. 



Sterilization: Not specified. 



Use: To study volutin production by yeast 



and Oidium Inctis. 

 Reference: Zikes (1922 p. 34). 



456. Gosio's Glycerol Aspartic Acid Solution 



Constituents : 



1. Water 800.0 cc. 



2. Glycerol 15.0 g. 



3. NaCl 2.5 g. 



4. NasCOs 2.5 g. 



5. K2HPO4 1.0 g. 



6. Aspartic acid 1-5 g. 



7. Grape sugar 50.0 g. 



8. CaCOa 20.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 and 



8 in 1. 

 Sterilization: Not specified. 

 Use: To study fermentation by Yihrio 



cholerae asiaticae. 

 Reference: Gosio (1894 p. 25). 



457. Gartner's Glycerol Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Asparagin 10 g. 



3. Glycerol 2.5 g. 



4. Dextrose 2.5 g. 



5. Potassium phosphate 1-0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Flask in 30.0 cc. lots. 

 Sterilization: Not specified. 



Use: To study nitrite oxidation by nitrate 

 fungus. The author reported trace of 

 nitrite after 4 weeks incubation. 



Reference: Gartner (1898 p. 4). 



458. Borrel, de Coulon, Boez and Quimaud's 



Glycerol Asparagin Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. FeS04 0.03 g. 



3. Potassium silicate 0.02 g. 



4. Glycerol 20.0 g. 



5. Mannitol 5.0 g. 



6. Glucose 5.0 g. 



7. Asparagin 4.5 g. 



8. (NH4)2C03 1.0 g. 



9. NaNOs 1.0 g. 



10. MgSOi 0.25 g. 



11. KHSO4 0.25 g. 



12. KH2PO4 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, S, 9, 10, 11 

 and 12 in 1. 



(2) Adjust to pH = 6.9. 

 Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. 

 Reference: Borrel, de Coulon, Boez and 

 Quimaud (1922 p. 389). 



459. Miiller, Thurgan and Osterwalder's 



Malate Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Malic acid 3.0 g. 



3. Asparagin 10.0 g. 



4. MgS04 0.4 g. 



5. K2HPO4 2.0 g. 



6. NaCl 2.0 g. 



7. Levulose 40.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 



