130 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation of Bacterium mannito- 

 poeum and bacteria from wines. There 

 is very little growth on this medium. 

 Organisms grow better if peptone and 

 potassium malate be added to the 

 medium. 



Reference: Midler, Thurgan and Oster- 

 walder (1912-13 p. 161). 



460. Maassen's Glucose Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. MgS04 (crystal- 

 line) 0.4 g. 



3. Asparagin 10.0 g. 



4. CaCl2 0.01 g. 



5. Na2HP04 2.0 g. 



6. NaCOs (crystalline). 2.5 g. 



7. Glucose 5.0 to 10.0 g. 



8. Malic acid 7.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 



7 and 8 in 1. 

 Sterilization: Method not given. 

 Use: As a general synthetic culture 



medium. 

 Reference; Klimmer (1923 p. 172). 



461. Clark and Lub's Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. NajHPOi (anhydrous) 7.0 g. 



or Na2HP04-2H20 8.8 g. 



3. KH phthalate 2.0 g. 



4. Aspartic acid 1.0 g. 



5. Glucose 4.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 800.0 cc. of 

 warm distilled water. 



(2) Cool the solution and make up to a 

 liter. 



(3) The hydrogen ion concentration of 

 the medium is fixed by the composi- 

 tion. It should be very close to pH 

 7.0, slightly red with phenol red. All 

 materials should be recrystallized or 

 if used from stock furnished by manu- 

 facturers, should be carefully exam- 

 ined. The di-sodium hydrogen phos- 

 phate may be used either as the 

 anhydrous salt obtained by dessica- 

 tion in vacuo in 100°C. or else as the 

 salt containing two molecules of 

 water of crystallization. This is 

 obtained by exposing the recrystal- 



lized Na2HP04l2H20 for two weeks. 

 Use 0.88 per cent of Na2HP042H20. 



Sterilization: Heat in an autoclave for 15 

 minutes after the pressure has reached 

 15 pounds, provided the total time of 

 e.xposure to heat is not more than one- 

 half hour. 



Use: Differentiation of colon-aerogenes 

 group. 



References: Clark and Lubs (1915, 160), 

 Levine (1921 p. 118), Committee Ameri- 

 can Public Health Assoc. (1925 p. 11). 



462. Remy and Sugg's Citrate Asparagin 

 Solution (Proskauer and Beck) 



Constituents : 



1. Water 



2. Glucose (0.1%) 



3. Asparagin (0.5%) 



4. Citric acid (0.075%)... 



5. K3PO4 (0.5%) 



6. MgSO4(0.25%) 



7. K2SO4 (0.25%) 



8. NaCl (0.15%) 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 



7 and 8 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of the tubercle bacilli. 

 Reference: Proskauer and Beck (1894 



p. 138). 



463. Uschinsky's Glycerol Asparaginate 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Glycerol 30.0-40.0 g. 



3. NaCl 5.0-7.0 g. 



4. CaCl2 0.1 g. 



5. MgS04 0.2-0.4 g. 



6. K2HPO4 2.0-2.4 g. 



7. Amm. lactate 6.0-7.0 g. 



8. Sodium asparaginate ... 3.4 g. 

 Preparation: (1) Dissolve 2, 3, 4, 5, 6, 



7 and 8 in 1. 



Sterilization: Not specified. 



Use: General culture medium. Author 

 reported growth was as good as in bouil- 

 lon. Diphtheria and tetanux toxin also 

 produced. Glucose increases production 

 of tetanus toxin. 



Variants : 



(a) The author added 10.0 to 20.0 g. 

 glucose. 



(b) Cramer specified the use of 10.0 g. 



