CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



131 



ammonium lactate, 3.4 g. asparagin, 

 40.0 g. glycerol, 5.0 g. NaCl, 0.2 g. 

 MgS04, but did not specify the 

 amount of K2HPO4. 



(c) Vaughan specified the use of 1.0 g. 

 NaCl, 0.4 g. K2HPO4 and 6.0 g. 

 ammonium lactate. 



(d) Hadley specified the use of 6.5 g. 

 ammonium lactate, 0.32 g. asparagin, 

 0.3 g. MgSOi, 2.5 g. K2HPO4 and 6.0 g. 

 NaCl. 



(e) Tanner used 3.4 g. asparagin, 10.0 g. 

 ammonium lactate, 5.0 g. NaCl, 

 0.2 g. MgS04-7H20, 1.0 g. K2HPO4 

 and 40.0 cc. glycerol. 



(f) Bezangon and Dopter and Sacquepee 

 used 3.0 to 4.0 g. asparagin. 



(g) Harvey used 6.0 g. NaCl, 0.1 g. CaCl, 

 0.3 g. MgS04, 2.5 g. K2HPO4, 8.0 g. 

 ammonium lactate, 3.5 sodium as- 

 paraginate, and 35.0 g. glycerol. 



(h) Harvey used 3.4 g. asparagin, 10.0 g. 

 ammonium lactate, 5.0 g. NaCl, 0.2 g. 

 MgS04, 0.1 g. CaCls, 1.0 g. K2HPO4, 

 and 40.0 g. glycerol, 

 (i) Harvey used 5.0 g. NaCl, 0.1 g. NaCl, 

 0.2 g. MgS04, 2.0 g. K2HPO4, 6.0 g. 

 ammonium lactate, 3.0 g. potassium 

 or sodium asparaginate and 30.0 g. 

 glycerol, 

 (j) Roux and Rochaix used 3.0 to 4.0 g. 

 asparagin instead of sodium asparag- 

 inate. 

 (k) Besson used 30.0 g. glycerol, 5.0 g. 

 NaCl, 0.2 g. MgS04, 2.0 g. K2HPO4, 

 6.0 g. ammonium lactate and 3.0 g. 

 asparagin. 

 References: Uschinsky (1893 p. 316), Cra- 

 mer (1894 p. 171), Vaughan (1896 p. 312), 

 Smith (1905 p. 197), Hadley (1907 p. 101), 

 Roux and Rochaix (1911 p. 246), Kalb and 

 Wasserman (1912 p. 394), Lohnis (1913 

 p. 44), Tanner (1919 p. 69), Bezangon 

 (1920 p. 116), Besson (1920 p. 37), Dopter 

 and Sacquepee (1921 p. 121), Harvey 

 (1921-22 pp. 102, 103), Klimmer (1923 

 p. 394). 



464. Sullivan's Glycerol Nitrate Asparagin 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Asparagin 15.0 g. 



3. K2HPO4 1.0 g. 



4. MgS04 



5. Ammonium lactate. 



6. NaCl 



7. KNO3 



0.2 g 

 0.8 g 

 1.0 g 

 0.3 g 



8. Glycerol 10.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 and 

 8 in 1. 



Sterilization: Not specified. 



Use: General culture medium. The author 

 reported that about 40 variaties developed 

 on this medium including chromogenic 

 bacteria and pathogenic forms. 



Variants : 



(a) Hadley substituted 25.0 g. glycerol 

 for 10.0 g. 



(b) Magnusson used 5.0 g. NaCl, 0.5 g. 

 ammonium lactate, 1.0 g. of sodium 

 asparaginate and 0.2 g. NaNOs. He 

 used this medium to study slime and 

 capsule production by Bacterium 

 lactis viscosum, and streptococci. 

 Slime and capsules were produced. 



Reference: Sullivan (1905-06 p. 115), Had- 

 ley (1907 p. 101), Magnusson (1918 p. 467). 



465. Hadley's Glycerol Asparagin Solution 

 No. V 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Ammonium lactate 5.0 g. 



3. Ammonium phosphate 10.0 g. 



4. Asparagin 10.0 g. 



5. reP04 0.2 g. 



6. Glycerin 40.0 g. 



7. MgS04 0.3 g. 



8. K2HPO4 3.0 g. 



9. NaCl 1.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 



8 and 9 in 1. 

 Sterilization: Not specified. 

 Use: Growth and toxin production of 



Bacillus diphtheriae. Author reported 



slight growth. 

 Reference: Hadley (1907 p. 101). 



466. Lowenstein's Glycerol Asparagin 

 Solution 



Constituents: 



1. Asparagin 6.0 g. 



2. Ammonium lactate 0.0 or 6.0 g. 



3. Sodium phosphate 



(neutral) 3.0 g. 



4. NaCl 6.0 g. 



5. Glycerin 40.0 g. 



