132 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation: (1) Original article does not 

 specify the use of water as a solvent. 

 The ammonium lactate may be omitted 

 if desired. 



Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. 



Reference: Lowenstein (1913 p. 591). 



467. Lockemann's Citrate Glycerol Aspara- 



gin Solution (Lowenstein) 



Constituents : 



1. Water 1000.0 cc. 



2. KH2PO4 5.0 g. 



3. MgS04 0.6 g. 



4. Magnesium citrate 2.5 g. 



5. Asparagin 5.0 g. 



6. Glycerol 20.0 g. 



7. Soda about 2.5 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of tubercle bacilli. 

 Variants : 



(a) Lockemann added 3.0 g. NaH2P04 

 and omitted the soda. 



(b) Harvey used 2.5 g. NaOH instead 

 of the soda. 



(c) Mueller used 2.6 g. magnesium citrate 

 and adjusted the reaction to pH = 

 7.0 using NaOH instead of soda. 



References: Lowenstein (1913 p. 592), 

 Lockemann (1919 p. 421), Harvey (1921- 

 22 p. 103), Mueller (1926 p. 3). 



468. Frouin's Glycerol Asparagin Solution 



(Bezangon) 



Constituents: 



1. Water 



2. MgS04 



3. Potassium phosphate. . 



4. Asparagin 



5. Lactose 



6. Sodium citrate 



1000.0 cc 

 1.0 g 

 1.0 g 

 5.0 g 

 6.0 g 

 3.0 g 



7. Glycerol 40.0 g 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of tubercle bacilli. 

 Reference: Bezan^on (1920 p. 548). 



469. Hadley's Urea Glycocoll Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Ammonium lactate 32.5 g. 



3. C&Clz 0.4 g 



4. Glycerin 170.0 g 



5. Glycocoll 5.0 g 



6. MgS04 1.6 g 



7. K2HPO4 12.5 g 



8. NaCl 30.0 g 



9. Urea 25.0 g 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 



8 and 9 in sufficient water to make 



1000.0 cc. 

 Sterilization: Not specified. 

 Use: Growth of Bacillus diphtheriae and 



study of to.xin production. 

 Reference: Hadley (1907 p. 101). 



470. Beijerinck's Urea Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KH2PO4 0.25 g. 



3. Asparagin 2.5 g. 



4. Urea 50.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Planosarcina ureae; 



Urobacillus p isteurii did not develop in 



this medium. 

 Reference: Beijerinck (1901 p. 53). 



471. Beijerinck's Urea Asparagin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KH2PO4 0.9 g. 



3. (NH4)2C03 0.2 g. 



4. Asparagin 0.25 g. 



5. Urea 1.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of urea organisms from 

 the soil, Urococcus ureae and Urobacillus 

 pasteurii. A soil culture will change all 

 the urea after incubation of 10 days. 



Reference: Beijerinck (1901 p. 53). 



472. Sears' Uric Acid Asparagin Solution 



Constituents: 



1. Water 



2. Asparagin. 



3. Na^CO, .. 



4. Na2HP04. 



5. MgS04.... 



6. CaCl2 



7. Uric acid. . 



1000.0 cc. 



10.0 g. 



2.5 g. 



2.0 g. 



traces 



traces 



0.902 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 

 in 1. 



