134 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



sodium asparaginate 0.5 g. 



K2HPO4 3.0 g. 



MgS04 0.4 g. 



KNO3 0.2 g. 



(d) Used 1.25 g. tryptophane in the basal 

 solution and added: 



histidine dichloride 1.25 g. 



glucosamine hydrochloride. . . 2.75 g. 



NaCl 2.50 g. 



K2HPO4 3.0 g. 



MgS04 0.4 g. 



(e) Used 0.6 g. tryptophane in the basal 

 solution and added: 



tyrosine 1.25 g 



leucin 3.00 g 



glutaminic acid hydrochloride 2.50 g 



glycocoll 0.85 g 



sodium asparaginate 1-00 g 



histidin dichloride 0.5 g 



creatin 0.2 g 



creatinin 0.08 g 



xanthin 0.05 g 



hypoxanthin 0.05 g, 



NaCl 4.0 g, 



K2HPO4 3.0 g 



MgS04 0.50 g 



glucose 1.5 g 



KNO3 0.20 g 



(f) Used 1.6 g. tryptophane in the basal 

 solution and added: 



glutaminic acid hydrochloride. 2.5 g. 

 glucosamine hydrochloride. . . 1.6 g. 



NaCl 2.5 g. 



K2HPO4 3.0 g. 



MgS04 0.4 g. 



(g) Used 1.0 g. tryptophane in the basal 

 solution and added: 



NaCl 2.50 g. 



K2HPO4 3.00 g. 



MgS04 0.4 g. 



(h) Used 1.6 g. tryptophane in the basal 

 solution and added: 

 glutaminic acid hydrochloride 2.5 g 

 glucosamine hydrochloride. . . 1.0 g 



histidine dichloride 1-20 g 



NaCl 2.5 g 



K2HPO4 3.0 g 



MgS04 0.4 g, 



Reference: Davis and Ferry (1919 pp. 226, 

 227, 228, 229, 230). 



475. Berthelot's Amino Acid Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 2.0g. 



3. MgS04 1.0 g. 



4. Glycocoll 1.5 g. 



5. Sodium asparaginate 1-0 g. 



6. Glutamic hydrochloride. . . 1.0 g. 



7. Alanine 1-0 g. 



8. Histidine chlorohydrate . . . 1.0 g. 



9. Tyrosine 0.5 g. 



10. Tryptophane 0.5 g. 



11. Leucine 0.6 g. 



12. Phenylalanine 0.6 g. 



13. Guanidine hydrochloride. . 0.4 g. 



14. Creatine 0.4 g. 



15. Glucosamine hydrochloride 0.4 g, 



16. Cystine 0.2 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 

 12, 13, 14, 15 and 16 in 1. 



(2) Adjustment of reaction not specified. 

 Sterilization: Sterilize with ether (method 



not given). 

 Use: Toxin production hy Proteus vulgaris. 



This medium will not permit successive 



growth of the organism. 

 Reference: Berthelot (1914 p. 917). 



476. Armand-Delille, et al, Glycocoll Argi- 

 nine Solution (Synthetic Medium 164) 



Constituents: 



1. Water 1000.0 cc. 



2. NaCl 5.0 g. 



3. KH2PO4 5.0 g. 



4. Magnesium citrate 2.4 g. 



5. Glucose 4.0 g. 



6. Glycerol 40.0 g. 



7. Glycocoll 4.0 g. 



8. Arginine 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Add 4.0 cc. of N/100 NaOH to (1) 

 after sterilization. 



Sterilization: Method not given. 



Use: Cultivation of Koch's bacilli. 



Medium gives good growth. 

 References: Armand-Delille, Mayer, 



Schaeffer and Terroine (1913 p. 274), 



Harvey (1921-22 p. 104). 



477. Armand-Delille, et al, Synthetic 

 Solution 104 



Constituents: 



1. Water 20.0 cc. 



2. NaCl 1.25 g. 



3. Magnesium citrate 0.6 g. 



