CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



135 



4. NaH.P04 



5. Glycocoll 



6. Aspartic acid 



7. Carnosine nitrate 



8. Creatinin 



9. Sarcosine 



10. Glucose 



11. Inosite 



1.25 g 

 0.50 g. 

 0.5 g. 

 0.1 g, 

 .. 0.1 g 

 0.1 g 

 0.5 g 

 .. 0.1 g 



12. Glycerol 10.0 g 



(Water given as 20.0 cc. In similar 

 media 389 and 391 water given as 

 250.0 cc.) 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 

 and 12 in 1. 



(2) Add 1.0 cc. of N/100 NaOH to (1) 

 after sterilization. 



Sterilization: Method not given. 

 Use: Cultivation of Koch's bacilli. 

 Reference: Armand-Delille, Mayer, 

 Schaeffer and Terroine (1913 p. 273). 



478. Armand-Delille, et al, Glycocoll 



Aspartic Acid Solution (Synthetic 



Medium 118) 



Constituents: 



1. Water 1000.0 cc. 



2. NaCl 5.0 g. 



3. Magnesium citrate 2.4 g. 



4. NaHiPO^ 5.0 g. 



5. Glycocoll 2.0 g. 



6. Aspartic acid 2.0 g. 



7. Glucose 2.0 g. 



8. Scombrine sulphate 2.0 g. 



9. Glycerol 40.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 1. 



(2) Add 4.0 cc. of N/100 NaOH to (1) 

 after sterilization. 



Sterilization: Method not given. 

 Use: Cultivation of Koch's bacilli. 

 References : Armand-Delille, Mayer, 

 Schaeffer and Terroine (1913 p. 273). 



47Q. Zipfel's Tryptophane Asparagin 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Asparagin 



3. Ammonium lactate 5.0 g. 



4. K,HP04 2.0 g. 



5. MgS04 0.2 g. 



6. Tryptophane (0.1%) 1.0 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5 and 6 in 1 (amount 

 of asparagin not specified). 



(2) Adjustment of reaction not specified. 



(3) Tube in 10.0 cc. lots. 

 Sterilization : Method not given. 

 Use: Study of indol production. 

 Variants : 



(a) Zipfel used 0.3 or 0.5% tryptophane 

 instead of 0.1%. 



(b) Barthel used 5.0 g. asparagin and 

 0.3 g. Tryptophane. 



(c) Barthel used 5.0 g. asparagin, 0.3 g. 

 tryptophane and omitted the 5.0 g. 

 ammonium lactate. 



References: Zipfel (1912 p. 74), Barthel 

 (1921 p. 85). 



SUBGROUP I-C. SECTION 6 



Liquid media, or basal solutions with 

 constituents of known chemical composi- 

 tion containing organic nitrogen other than 

 amino acids; amino acids not present. 

 Al.'* Organic nitrogen supplied as cyanogen 



or its compounds. 

 Bi. Nitrogen supplied as cyanamide. 

 Kappen's Glucose Cyanamide Solu- 

 tion 480 



Vierling's Glucose Cyanamide Solu- 

 tion 481 



Bs. Nitrogen supplied as dicyanamide. 

 Perotti's Dicyandiamide Solution . . , 482 

 Perotti's Glucose Dicyandiamide 



Solution 483 



Kappen's Glucose Dicyandiamide 



Solution 484 



Bs. Nitrogen supplied as ferrocyanamide. 

 Doryland's Basal Ferrocyanide Salt 



Solution 485 



Bokorny's Sucrose Ferrocyanide 



Solution 486 



A2.'* Organic nitrogen supplied as urea. 

 Bi. Incomplete or basal solutions, em- 

 ployed with additional nutrients. 

 Stutzer and Hartleb's Basal Urea 



Solution 487 



Beijerinck's Basal Urea Salt Solution. 488 

 Waksman's Basal Urea Salt Solu- 

 tion 489 



Christensen's Basal Urea Salt Solu- 

 tion 490 



Bj. Complete media. 



Ci. Containing no additional organic com- 

 pounds. 



*See also A3, A4, As and As on page 136. 



