136 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Fremlin's Urea Solution 491 



Wherry's Urea Solution 492 



Evans' Urea Solution 493 



Percival's Urea Solution 494 



Ca. Containing additional organic com- 

 pounds. 



Di. Carbohydrates added. 

 Murray's Glucose Urea Solution .... 495 

 Vierling's Glucose Urea Solution. . . . 496 



Thomas' Sucrose Urea Solution 497 



Hugounenq and Doyon's Sucrose 

 Urea Solution 498 



D2. Alcohols added. 



Hadley's Glycerol Urea Solution 499 



Harvey's Glycerol Urea Solution. . . . 500 



A3. Organic nitrogen supplied as uric acid. 

 Koser's Glycerol Uric Acid Solution. . 501 

 Lohnis' Uric Acid Solution 502 



A4. Organic nitrogen supplied as amines 

 and amides. 



Bokorny's Amine Solution 503 



Omeliansky's Amine Solution 504 



Beijerinck and Minkman's Glucose 

 Amine Solution 505 



As. Organic nitrogen supplied as hippuric 

 acid. 

 Went's Basal Hippuric Acid Solution. 506 



Stapp's Hippurate Solution 507 



Lohnis' Hippurate Solution 508 



Ae. Organic nitrogen supplied other than 

 Ai to As. 



Stoklasa's Glucose Lecithin Solution. 509 

 Stoklasa's Nucleic Acid Solution. . . . 510 



Went's Basal Creatin Solution 511 



Stutzer's Glucose Creatin Solution. . 512 



Meyers' Taurine Solution 513 



Wherry's Theobromin Solution 514 



Bokorny's Picric Acid Solution 515 



480. Kappen's Glucose Cyanamide Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Glucose (1.0%) 10.0 g. 



3. KH2PO4 (0.5%) 5.0 g. 



4. Cyanamide (0.5%,) 50 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: To study decomposition of cyanamide 

 by Penicillium brevicaule, Stysanus 

 stemonitis and Cladosporium. Cyana- 

 mide in 2.0% strength was toxic for some 

 of the types studied. Using 0.5% cyana- 

 mide and varying the dextrose content, 

 the most NH3 was produced from the 

 cyanamide with 0.5 to 1.0% dextrose. 



Variants: 



(a) The author used 1.0 or 2.0% cyana- 

 mide instead of 0.5%. 



(b) The author used 0.1, 0.5 or 1.0% 

 glucose with 0.5% cyanamide. 



Reference: Kappen (1910 p. 635). 



481. Vierling's Glucose Cyanamide Solution 

 Constituents: 



1 . Water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. CaCl2 0.1 g. 



4. MgS04 0.1 g. 



5. FeCls trace 



6. NaCl trace 



7. Calcium cyanamide 0.2 g. 



8. Glucose 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of m3^cobacteria. 

 Reference: Vierling (1920 p. 202). 



482. Perotti's Dicyandiamide Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. NaCl 0.5 g. 



4. CaS04 0.5 g. 



5. MgS04 0.5 g. 



6. Iron chloride drops 



7. C2N4H4 (dicyandiamide)... 0.5 g. 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Distribute in flasks. 

 Sterilization: Sterilize on 3 successive days 



at 120^C. for 10 minutes, each day. 



Use: To study influence of dicyandiamide 

 on field plants. Author reported that the 

 nitrogen content was increased. As a 

 control KNO3 was used instead of 

 dicyandiamide. 



Reference: Perotti (1908 p. 225). 



483. Perotti's Glucose Dicyandiamide 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. dicyandiamide 2.0 g. 



3. Dextrose 0.1 g. 



4. K2HPO4 0.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. (Use tap 

 water or distilled water, with traces 

 of NaCl, Na2S03 and CaClj.) 



