CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



137 



(2) Distribute 200.0 cc. of 1 in a one liter 

 flask. 



(3) Inoculate with 0.2 g. of garden earth. 

 Sterilization: Not specified. 



Use : Cultivation of dicyandiamide bacteria 



from the soil. 

 Variants: The author gives the following 



solution: 



1. Water 1000.0 cc. 



2. Potassium phosphate 1.0 g. 



3. CaCl. 0.1 g. 



4. MgS04 0.3 g. 



5. NaCl 0.1 



6. Iron chloride 0.01 



7. Glucose (0.5%) 5.0 g. 



8. Dicyandiamide 0.1, 1.0, 5.0 or 10.0%. 

 Reference: Perotti (1908, vol. 21, p. 207), 



(1908, vol. 20, p. 516). 



484. Kappen's Glucose Dicyanamide 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 (0.05%) 5.0 g. 



3. MgS04 (0.5%) 5.0 g. 



4. Dicyanamide (1.0%) 10.0 g. 



5. Glucose (10.0%) 100.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: To study decomposition of cyana- 

 mide by Penicillium bremcaule, Stysanus 

 stemonitis and Cladosporium. 



Reference: Kappen (1910 p. 637). 



485. Doryland's Basal Ferrocyanide Salt 

 Solution 



Constituents: 



1. Distilled water 500.0 cc. 



2. HCl dilute 



3. MgS04 0.5 g. 



4. CaO 0.01 g. 



5. Fe2(S04)., 0.01 g. 



6. MgS04 0.01 g. 



7. KGFe2(CN)i2, amount not given. 



8. H2SO4 



9. H3PO4 



10. N/0.2578 NaOH 



11. N/0.6205 KOH 

 Preparation : 



(1) Dilute the HCl so that 1.0 cc. is not 

 quite neutralized by 1.0 cc. of silicate 

 solution made by dissolving 24.0 g. 

 KSiOi and 8.4 g. NajSiOa in 500.0 cc. 



distilled water, using phenolphthalein 

 as indicator. 



(2) Add to HCl the following salts: 



MgS04 0.5 g. 



Fe2(S04)3 0.01 g. 



CaO 0.01 g. 



MnS04 0.01 g. 



K6Fe2(CN)i2, amount not given. 



(3) Standardize (2) against silicate solu- 

 tion so that 1.0 cc. is equivalent to 

 1.0 cc. using methyl orange as indi- 

 cator. 



(4) Standardize a solution of H2SO4 in 

 same way as HCl omitting salts. 



(5) Standardize H3PO4 in similar manner 

 as HCl omitting the salts and using 

 phenolphthalein as indicator. 



(6) ]\Iix the acids in the following ratio: 



HCl 153.5 cc. 



H2SO4 77.0 cc. 



H3PO4 116.0 cc. 



(7) Mix equal quantities of N/0.6205 

 KOH and N/0.2578 NaOH. 



(8) 1.0 cc. of (7) should neutralize 1.0 cc. 

 of (6), phenolphthalein as indicator. 



(9) Draw acid and base solution into 

 separate burettes and allow to stand 

 several hours to sterilize. 



Sterilization: Method specified in prepa- 

 ration. 



Use: Synthetic culture medium. 



Added nutrients: Acetic acid or glucose 

 were used as additional carbon source. 

 When using acetic acid mix with H3PO4 

 in step (5). When using glucose add a 

 sufficient quantity of a sterile aqueous 

 glucose solution to a mixture of equal 

 parts of (6) and (7) so that there will be 

 10.0 g. of glucose per liter of solution. 



Reference: Doryland (1916 p. 148). 



486. Bokomy's Sucrose Ferrocyanide 

 Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Sucrose 30.0 g. 



3. Potassium ferrocvanide 

 (K4Fe(CN),) ^ 60 g. 



4. K2HPO4 1.0 g. 



5. MgS04 0.32 g. 



6. CaCl2 0.08 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 



