138 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation of Schizomycetes. Au- 

 thor reported that mold spores did not 

 develop in this medium. Schizomycetes 

 developed producing lactic acid. The 

 acid produced may decompose the 

 K4Fe(CN)o. Potassium ferrocyanide was 

 a suitable nitrogen source for schizomy- 

 cetes but not for molds. 



Variants : Bokorny also used the following 

 solution: 



1. Water 1000.0 cc. 



2. Sucrose 20.0 g 



3. Potassium ferrocyanide . . 2.0 g 



4. K2HPO4 2.0 g 



5. MgS04 0.052 g 



6. CaCl2 0.012 g 



Reference: Bokorny (1917 pp. 343, 344). 



487. Stutzer and Hartleb's Basal Urea 

 Solution 



Constituents: 



1. Water 



2. Urea 



3. Glycerin 



4. Potassium phosphate. . 



. 1000.0 cc. 



. 20.0 g. 



. 10.0 g. 



1.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of the organisms from 



cases of foot and mouth disease. 

 Added nutrients and variants: 



(a) The authors used the basic solution 

 as a medium. 



(b) Sohngen used 5.0% urea and 0.025 

 K2HPO4 as the basic solution and 

 added 1.0% of one of the following: 

 ammonium oxalate 



sodium acetate 



Rochelle salt 



ammonium citrate 



ammonium malate 



He used the media to study urea 



decomposition by bacteria from the 



soil. 



(c) Sohngen used 0.05% K2HPO4 and 

 2.0% urea in the basic solution and 

 added 5.0 to 61.5 mg. of nitrogen in 

 the form of asparagin or ammonium 

 malate to study urea decomposition 

 by Urobacillus Jakschii. 



(d) Sohngen used 0.05%, K2HPO4 and 

 5.0% urea in the basic solution and 

 added 5.0 to 61.5 mg. of nitrogen in 

 the form of asparagin or ammonium 

 malate to study urea decomposition 

 by Bacillus erythrogenes. 



(e) Sohngen used 0.05% K2HPO4 and 

 3.0% urea in the basic solution and 

 added 1.0% of calcium malate or cal- 

 cium citrate to study urea decomposi- 

 tion by bacteria from the soil. 



(f) Sohngen used a 0.5% urea and 0.05% 

 K2HPO4 solution without additions 

 to cultivate Bacillus erythrogenes. 



(g) Percival used 5.0% urea and 0.25% 

 K2HPO4 in the basic solution and 

 added 1.0% (NH4)2S04 to enrich 

 urea splitting organisms found in the 

 soil and in urine. 



(h) Giltner used 5.0% urea and 0.05% 



K2HPO4 in the basic solution and 



added 0.5 to 1.0% of one of the 



following: 



ammonium malate 



calcium malate 



calcium citrate 



ammonium citrate 



He used the media to show urea 



decomposition. 

 References: Stutzer and Hartleb (1897 

 p. 404), Sohngen (1909 pp. 91, 93, 94, 95), 

 Percival (1920 p. 225), Giltner (1921 p. 

 314). 



488. Beijerinck's Basal Urea Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Urea 5.0 g. 



3. KH2PO4 0.025 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 1.0% of one of the added nu- 

 trients. 



Sterilization: Not specified. 

 Use: Cultivation of urea organisms from 

 the soil, Urobacillus pasteurii, Urococcus 

 ureae. Author reported that about 2.0% 

 of the urea was changed by a soil culture 

 using o.xalate as carbon source, 2.0% 

 using sodium acetate, 2.0% using Seig- 

 nette salt, 3.0% using ammonium citrate 

 and 4.% using ammonium malate. 

 Added nutrients and variants: 



(a) 1.0% of one of the following carbon 

 sources were added: 



ammonium oxalate 1-0 g. 



sodium acetate 

 Seignette salt 



(potassium sodium tartrate) 1.0 g. 



Ammonium citrate 1-0 g. 



Ammonium malate 1-0 g. 



