CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



141 



3. KH2PO4 2.25 g. 



4. MgS04 0.45 g. 



5. Urea 1.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Prepare a 20.0% urea solution in 

 water. 



(3) Add 20.0 cc. of sterile (2) to cool 

 sterile (1) under aseptic conditions. 



Sterilization: Sterilize (1) at 120°C. Steril- 

 ize (2) by filtration. 

 Use: Cultivation of yeast. 

 Reference: Thomas (1919 p. 800). 



498. Hugounenq and Doyen's Sucrose Urea 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol 45.0 g. 



3. NaCl 7.0 g. 



4. Ammonium lactate 10.0 g. 



5. CaClo 0.1 g. 



6. MgS04 0.2 g. 



7. Potassium biphosphate 2.0 g. 



8. Uric acid 0.02 g. 



9. Urea 5.0 g. 



10. Sucrose 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 8, 



9 and 10 in 1. 

 Sterilization: Not specified. 

 Use: General culture medium. Reported 



as not being a suitable medium for 



diphtheria bacilli. 

 Reference: Hugounenq and Doyon (1896 



p. 401). 



499. Hadley's Glycerol Urea Solution 



Constituents: 



1. Distilled water 100.0 cc. 



2. Ammonium lactate 3.2 g. 



3. CaCl, 0.05 g. 



4. Glycerol 15.0 g. 



5. MgS04 0.15 g. 



6. K0HPO4 1.25 g. 



7. NaCl 3.0 g. 



8. Urea 1.6 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 



7 and 8 in sufficient water to make 



100.0 cc. 

 Sterilization: Not specified. 

 Use: Growth of Bacillus diphlheriae and 



production of toxin. 

 Reference: Hadley (1907 p. 101). 



500. Harvey's Glycerol Urea Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NaCl 0.02 g. 



3. MgSOi 0.02 g. 



4. K2HPO4 0.5 g. 



5. FeCla trace 



6. Glycerol 10.0 g. 



7. Urea 1.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 



Sterilization: Not given. 



Use: General culture medium. As other 

 sources of carbon, saccharose, glucose, 

 mannite, calcium lactate, etc., were used. 

 As other sources of nitrogen, sodium 

 hippurate, uric acid, asparagin, ammo- 

 nium sulphate, ammonium acetate, am- 

 monium butyrate, and ammonium lac- 

 tate were used. 



Reference: Harvey (1921-22 p. 104). 



501. Koser's Glycerol Uric Acid Solution 



Constituents: 



1. Distilled water (NH3 free). 1000.0 cc. 



2. NaCl 5.0 g. 



3. MgS04 0.2 g. 



4. CaCU 0.1 g. 



5. K2HPO4 1.0 g. 



6. Glycerol 30.1 g. 



7. Uric acid 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Adjustment of reaction not given. 



(3) Tube. 



Sterilization: Tube and sterilize at 13 to 

 15 pounds extra pressure for 15 minutes. 

 A slight turbidity appears after auto- 

 claving but upon cooling the solution 

 becomes clear. 



Use: B. aerogenes soon produces a dense 

 clouding. B. coli fails to develop. Care 

 must be taken to keep the medium 

 ammonia free. 



Variants : Ecker and Morris used 0.2 g. uric 

 ac'd instead of 0.5 g. 



References: Koser (1918 p. 377), Levine 

 (1920 p. 118), Harvey (1921-22 p. 104), 

 Ecker and Morris (1924 p. 480), Com. 

 American Public Health Asso. (1925 

 p. 112). 



