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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



502. Lohnis' Uric Acid Solution 



Constituents : 



1. Water (tap) 1000.0 cc. 



2. Uric acid 3.0 g. 



3. K2HPO4 0.5 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Distribute in small Erlenmeyer flasks. 

 Sterilization: Not specified. 



Use: Cultivation of B. ^Morescens and uric 



acid bacteria. 

 Reference: Lohnis (1913 p. 96). 



503. Bokorny's Amine Solution 



Constituents : 



1. Water 1000.0 cc. 



2. K.HPO4 2.5 g. 



3. MgP04 0.2 g. 



4. CaCh 0.05 g. 



5. Methyl amine hydro- 

 chloride (0.5%) 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 



Sterilization: Not specified. 



Use: To study the constituents essential 



for growth of molds. Molds grew readily 



on this medium. 

 Variants: The author used the following 



amines and amides instead of methyl 



amine hydrochloride: 



(a) 1.0% propyl amine hydrochloride. 

 Certain molds developed on this 

 medium. 



(b) 0.5% oxamide. Yeast did not grow 

 in this medium. 



(c) The salt concentration was changed 

 and materials added. The medium 

 used had the following composition: 



1. Water 1000.0 cc. 



2. Trimethyl amine 



acetic acid 5.0 g. 



3. Sucrose 50.0 g. 



4. K2HPO4 2.0 g. 



5. MgSOi 0.2 g. 



6. CaCl2 0.2 g. 



7. H3PO4 0.0 or 1.0% 



Trimethyl amine served as a nitrogen 

 source for bacteria and without the 

 H3PO4 Schizomycetes developed then 

 the molds. In the presence of H3PO1 

 molds developed at once. 



Reference: Bokorny (1917 pp. 337, 338). 



504. Omeliansky's Amine Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Potassium phosphate 10 g. 



3. MgSOi 0.5 g. 



4. NaCl 2.0 g. 



5. Methyl amine 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Adjustment of reaction not given. 



(3) Add the usual amount of sterile 

 MgCOs to the sterile fluid. 



Sterilization: Sterilize by heating at 115°C. 



for 30 minutes. 

 Use: To study nitrification by nitrite 



formers. The methyl amine or dimethyl 



amine was not oxidized. 

 Variants: The author used 0.5 g. dimethyl- 



amine instead of methyl amine. 

 Reference: Omeliansky (1899 p. 485). 



505. Beijerinck and Minkman's Glucose 

 Amine Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose amine (0.5%) 5.0 g. 



3. KNO3 10.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use : To study denitrification by soil forms, 



Bacillus nitroxus. 

 Reference: Beijerinck and Minkman (1910 



p. 37). 



506. Went's Basal Hippuric Acid Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Hippuric acid (0.66%) 6.6 g. 



Preparation : 



(1) Prepare a 0.66% solution of hippuric 

 acid. 



(2) Prepare a 5.0% solution of one of the 

 added nutrients. 



(3) Mix equal volumes of (1) and (2). 

 Sterilization: Not specified. 



Use: Cultivation of Monilia sitophila 

 (Mont.) Sacc. Maltose, glucose, lactose, 

 sucrose and glycerol was the order of 

 vigor of growth for the carbon sources 

 studied. 

 Added nutrients: One of the following 

 carbon sources was employed: 

 maltose glucose 



lactose glycerol 



sucrose 

 Variants: No additional carbon source was 



added. No growth occurred. 

 Reference: Went (1901 p. 593). 



