CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



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507. Stapp's Hippurate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KH2PO4 1.25 g. 



3. MgSOi 0.625 g. 



4. Sodium hippurate 3.1 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Distribute in 50.0 cc. lots in 200.0 cc. 

 Erlenmeyer flasks. 



Sterilization: Not specified. 



Use: Isolation of uric acid splitting bac- 

 teria from feces and soil. Bac. cobayae, 

 Bac. capri, Bad., guano, Bac. musculi, 

 Bac. hollandicus. 



Reference: Stapp (1920 p. 4). 



508. Lohnis' Hippurate Solution 



Constituents: 



1. Water (tap) 1000.0 cc. 



2. Sodium hippurate 3.0 g. 



3. K2HPO4 0.5 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Distribute in small Erlenmeyer flasks. 

 Sterilization: Not specified. 



Use: Cultivation of hippuric acid bacteria. 

 Author reported that fermentation took 

 place rather slowly when the medium was 

 inoculated with manure. 



Reference: Lohnis (1913 p. 96). 



509. 



Stoklasa's Glucose Lecithin 

 Solution 



Constituents: 



1. Distilled water 



2. NH4NO3 



3. K2SO4 



4. MgClj 



5. NaCl 



6. Al2(S04)3 + FeS04 



8000.0 cc 

 20.0 g 

 8.0 g 

 4.0 g 

 1.0 g 

 0.12 g 



7. d-glucose 80.0 



8. lecithin 8.0 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Distribute into large 2 liter Erlen- 

 meyer flasks in 250.0 cc. lots. 



(3) Adjustment of reaction not specified. 



(4) After sterilization add 0.25 g. leci- 

 thin to each flask. Heat at 45 to 

 50° for 20 hours. 



Sterilization: Sterilize thoroughly (method 

 not given). 



Use: To study the cycle of the phosphate 

 ion in the soil. Author used Bacillus 

 mycoides, Bacillus subtilis, Bacillus pro- 

 teus vulgaris. Amount of phosphoric 

 anhydride formed by each organism was 

 about the same after 60 days. If the 

 NH4NOJ be omitted the nitrogen con- 

 tent is too low to give good growth. 



Variants: Author omitted the NH4NOS 

 and used only 0.8 g. NaCI instead of 1.0 g. 



Reference: Stoklasa (1911 pp. 423, 424). 



510. Stoklasa's Nucleic Acid Solution 



Constituents: 



1. Distilled water 8000.0 cc. 



2. K2SO4 8.0 g. 



3. MgCli 4.0 g. 



4. NaCl 1.0 g. 



5. Al2(S04)3 + FeS04 0.12 g. 



6. Nuclein acid 8.0 g. 



7. Glucose 80.0 g. 



Preparation: 



(1) Prepare nucleic acid from j^east by 

 Herlant's method (method not given) . 



(2) Dissolve 2, 3, 4 and 5 in 1. 



(3) Distribute in 250.0 cc. lots in large 

 Erlenmeyer flasks. 



(4) Add 0.25 g. nucleic acid and 2.5 g. 

 d-glucose to each flask. 



(5) Heat at 45 to 50°C. for 20 hours. 

 Sterilization: Method not given. 



Use: To study the cycle of the phosphate 

 ion in the soil. Author used Bacillus 

 mycoides, Bacillus subtilis, Bacillus 

 proteus vulgaris. Bacillus mycoides util- 

 ized the largest amount of phosphorous. 



Reference: Stoklasa (1911 p. 428). 



511. Went's Basal Creatin Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Creatin (0.66%) 6.6 g. 



Preparation : 



(1) Prepare a 0.66% creatin solution. 



(2) Prepare a 5.0% solution of one of the 

 added carbohydrates. 



(3) Mix equal volumes of (1) and (2). 

 Sterilization: Not specified. 



Use: Cultivation of Monilia sitophila 

 (Mont.) Sacc. Maltose, glucose, lactose, 

 sucrose and glycerol was the order of 

 vigor of growth for the carbon sources 

 studied. 



