144 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Added nutrients: One of the following car- 

 bon sources was employed: 



maltose sucrose 



glucose glycerol 



lactose 

 Variants: The author used the basic solu- 

 tion without any added materials. 

 Reference: Went (1901 p. 593). 



512. Stutzer's Glucose Creatin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Creatin 2.5 g. 



3. K2HPO4 1.0 g. 



4. KNO3 3.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Reaction to be slightly alkaline. 



(3) Distribute in 10.0 cc. lots. 

 Sterilization: Method not given. 



Use: To study denitrification by B. agilis, 

 B. nitrovorus, B. Stutzeri, B. Hartlebi. 

 Nitrate is not reduced. 



Variants : The author added 10.0 g. glucose. 



Reference: Stutzer (1901 p. 83). 



513. Myers' Taurine Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Taurine 5.0 g. 



Preparation : 



(1) Prepare taurine according to Hawk 

 (1918). 



(2) Dissolve 2 in 1. 



(3) No adjustment of reaction specified. 



(4) Suspend a sterile piece of filter paper, 

 that has been soaked in 10.0% solu- 

 tion of lead acetate, in each flask. 



Sterilization : Filter thru a Berkefeld filter. 

 Use: To study HoS production. 

 Variants: Myers added 10.0 g. glucose. 

 Reference: Myers (1920 p. 249). 



514. Wherry's Theobromin Solution 

 Constituents: 



1. Redistilled water 1000.0 cc. 



2. NaoCOs 1.0 g 



3. CaCls 1.0 g 



4. KCl 1.0 g 



5. MgS04 1.0 g 



6. Theobromin 0.05 g 



7. KH2PO4 2.0 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Adjustment of reaction not given. 



Sterilization: Sterilize in autoclave, time 

 not given. 



Use: To study acid proofness of B. tuber- 

 culosis. On 19th day rods and diplococ- 

 coids were non acid proof. No growth 

 using caffein instead of theobromin. 



Reference: Wherry (1913 p. 151). 



515. Bokorny's Picric Acid Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Ca(N03) 2 (0.02%) 0.2 g. 



3. KH2PO4 (0.02%) 0.2 g. 



4. MgS04 (0.02%) 0.2 g. 



5. Picric acid (0.5%) 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of fungi, algae, amobae 

 and infusoria. No growth with 0.5% 

 or 0.1% picric acid. 



Variants: Bokorny used 0.1, 0.05, or 0.01% 

 picric acid instead of 0.5%. 



Reference: Bokorny (1917 p. 346). 



SUBGROUP I-C. SECTION 7 



Liquid media or basal solutions, contain- 

 ing organic carbon only as commercial 

 digests; additional material, if any, in- 

 organic. 

 Ai. Not containing peptone. 



Robinson and Rettger's Opsine Solu- 

 tion 516 



Linde's Nahrstoff-Heyden Solution. . 517 



Lichtenstein's Cenovis Solution 518 



A2. Containing peptone. 



Bi. Containing no added salts. (Exclusive 



of indicators or dyes.) 



Dunham's Peptone Solution (Comm. 

 A. P. H. A.) 519 



Levine's Crystal Violet Peptone Solu- 

 tion 520 



Signorelli's Indicator Peptone Solu- 

 tion 521 



Steam's Gentian Violet Peptone 

 Solution 522 



Rivas' Trypsinized Peptone Solu- 

 tion 523 



B2. Containing added salts. 



Ci. Containing salts of monovalent cations 



only. 

 Di. Only one salt added 

 El. Sodium chloride only salt added. 

 Fi. No indicator added. 



Dunham's Peptone Solution 524 



Huss' Peptone Solution 525 



