CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



145 



Nencki's Peptone Solution 526 



Brussoff's Peptone Solution 527 



Redfield's Peptone Solution (Meyers). 528 

 Berman and Rettger's Peptone Solu- 

 tion 529 



Migula's Peptone Solution 530 



Heap and Cadness' Basal Peptone 



Solution 531 



F2. Indicator added. 



Browning, Gilmour and Mackie's 



Brilliant Green Peptone Solution. . 532 

 Harvey's Telluric Acid Peptone Solu- 

 tion 533 



Smith's Rosalie Acid Peptone Solu- 

 tion 534 



Bronfenbrenner's China Blue Pep- 

 tone Solution 535 



E2. Potassium (or sodium) nitrate onlj' 

 salt added. 



Diedudonne's Nitrate Peptone Solu- 

 tion 536 



Harvey's Nitrate Peptone Solution. . 537 

 E3. A salt other than Ei or E2 added. 

 Cohen and Clark's Phosphate Pep- 

 tone Solution 538 



Enlow's Basal Agar Peptone Solu- 

 tion 539 



Beijerinck's Ammonium Sulphate 



Peptone Solution 540 



Redfield's Peptone Solution (Tan- 

 ner) 541 



Nicolle, Raphael and Debains' Pep- 

 tone Solution 542 



Levine's Boric Acid Peptone Solu- 

 tion 543 



D2. More than one salt added. 

 El. Containing phosphates. 

 DeBord's Phosphate Peptone Solu- 

 tion 544 



Stutzer's Nitrate Peptone Solution.. 545 

 Kendall, Day and Walker's Fat Free 



Peptone Solution 546 



Kligler's Peptone Solution 547 



Klecki's Basal Ammonium Phosphate 



Peptone Solution 548 



E2. Not containing phosphates. 



Pergola's Nitrate Peptone Solution. . 549 

 Klimmer's Nitrate Peptone Solution. 550 

 Bleisch's Nitrate Peptone Solution. . 551 

 Logie's Nitrite Peptone Solution. . . . 552 

 C2. Containing salts of divalent cations. 

 Di. Containing calcium. 



Gottheil's Peptone Solution 553 



Guyenot's Peptone Solution 554 



Will's Peptone Solution 555 



Gersbach's Trypsinized Peptone 



Solution 556 



D2. Not containing calcium. 

 El. Containing iron salts. 



Kappen's Peptone Solution 557 



Percival's Ammonium Sulphate Pep- 

 tone Solution 558 



E2. Not containing iron salts. 



Fremlin's Ammonium Sulphate Pep- 

 tone Solution 559 



Mortensen's Peptone Solution 560 



Kligler's Nitrate Peptone Solution. . 561 

 Percival's Phosphate Peptone Solu- 

 tion 562 



Capaldi and Proskauer's Peptone 



Solution 563 



Ritter's Peptone Solution 564 



Speakman's Basal Peptone Solution 

 (McCoy et al.) 565 



516. Robinson and Rettger's Opsine 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Opsine (1.0%) 10.0 g. 



Preparation : 



(1) Dissolve 1.0% opsine in water over a 

 free flame. 



(2) Adjust the reaction to -|-0.6 to phenol- 

 phthalein. 



(3) Boil two or three minutes. 



(4) Filter and distribute in 20.0 cc. lots 

 in 150.0 cc. Erlenmeyer flasks. 



Sterilization: Sterilize at 12 to 14 pounds 



pressure for 15 minutes. 

 Use: Cultivation of B. diphtheriae and 



study of to.xin production. Using 2% 



opsine M.L.D. = 0.7 cc. 

 Variants : 



(a) Used 2.0% opsine. 



(b) Used 1.0% or 2.0% opsine with 1.0% 

 glucose, and 0.5% NaCl. 



(c) Used 1.0% or 2.0% opsine with 0.5% 

 NaCl. 



Reference: Robinson and Rettger (1917 

 p. 363). 



517. Linde's Nahrstofif Heyden Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Niihrstoff Heyden 5.0 g. 



Preparation: (1) Dissolve 2 in 1. 

 Sterilization: Not given. 



