146 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation of Cladothrix. Author 

 reported good growth for 2 or 3 days. 

 Then growth stopped. 



Reference: Linde (1913 p. 386). 



518. Lichtenstein's Cenovis Solution 



Constituents : 



1. Water 



2. Cenovis 

 Preparation : 



(1) Add cenovis (a fine yellow yeast 

 preparation) to water (amount not 

 given). Mix well. 



(2) Allow to stand at room temperature 

 for 30 minutes and then steam for 

 2 hours. 



(3) Neutralize with soda solution and 

 sterilize pin like steamer for another 

 hour. 



(4) Filter. Filtrate is clear golden yel- 

 low fluid. 



Sterilization: Not specified. 



Use: General culture medium. Substitute 



for meat peptone. 

 Reference: Lichtenstein (1923 p. 390). 



519. Dunham's Peptone Solution (Committee 

 A. P. H. A.) 



Constituents: 



1. Water (tap) 1000.0 cc. 



2. Peptone 10.0 g. 



Preparation : 



(1) Weigh a sauce pan and measure 

 1000.0 cc. of tap water into it. 



(2) Dissolve 10.0 g. of peptone in (1) 

 when hot. 



(3) Make up the loss due to evaporation 

 by adding water. 



(4) Filter twice thru the same filter. 



(5) Distribute into tubes. 

 Sterilization: Autoclave for 10 minutes 



at 120°C. 

 Use: General culture medium. Indol test. 

 Variants : 



(a) Buhlert and Fickendey used 1.5% 

 peptone solution to show peptone 

 decomposition by bacteria from the 

 soil. 



(b) Molisch used a 0.05% manganese 

 peptone solution for the cultivation 

 of iron bacteria Leptothrix ochraceae, 

 etc. 



(c) Remy and Rosing used a 0.8% solu- 

 tion of Witte's peptone or a 1.0% 



Merck peptone solution to study 

 decomposition of peptone by bac- 

 teria from the soil, 

 (d) Linde used a 0.1 or 0.5% peptone 

 solution to cultivate Cladothrix. 

 Good growth was obtained for 2 or 

 3 days. 

 References: Committee A. P. H. A. (1905 

 p. 110), Heinemann (1905 p. 25), Buhlert 

 and Fickendey (1906 p. 400), Molisch 

 (1910 p. 36), Remy and Rosing (1911 p. 39), 

 Linde (1913 p. 386), Tanner (1919 p. 47). 



520. Levine's Crystal Violet Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (1.0%) 10.0 g. 



3. Crystal violet 

 Preparation : 



(1) Dissolve 2 in 1. 



(2) Add varying amounts of crystal 

 violet. 



Sterilization: Not specified. 



Use: Differentiation of Bad. coli and Bad. 

 aerogenes. The author reported that 

 1-100,000 crystal violet prevented growth 

 of Bod. coli, while Bad. aerogenes grew 

 heavily. Decreasing the concentration 

 of peptone to 0.5% increased markedly 

 the inhibitory action of the dye. 



Reference: Levine (1911 p. 22). 



521. Signorelli's Indicator Peptone Solution 



Constituents: 



1. Peptone water 1000.0 cc. 



2. Dahlia (1.0% soln.) 5.0 cc. 



Preparation : 



(1) Exact composition of peptone water 

 not given. 



(2) Adjust (1) to neutral. 



(3) Tube in 10.0 cc. lots. 



(4) Add 0.05 cc. of 1.0% dahlia solution 

 to each tube. 



Sterilization: Not specified. 



Use: To show adsorption of dye by cholera 

 vibrio. The author reported that the 

 vibrios developed rapidly and fell to the 

 bottom of the tube. They were highly 

 colored with dahlia and the medium was 

 decolorized. With the other dyes the 

 vibrio developed rapidly but did not 

 color so strongly. The media were not 

 decolorized. 



