CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



147 



Variants: Signorelli prepared 1.0% solu- 

 tions of erythrosin or sapronia and 

 added 1.0 cc. of one of the solutions to 

 each tube of culture medium. 



Reference: Signorelli (1912 p. 472). 



522. Steam's Gentian Violet Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc 



2. Peptone 20.0 g. 



3. Gentian violet 1:200,000 



Preparation : 



(1) Dissolve 2 in 1. 



(2) Add 1 to 200,000 parts gentian violet. 



(3) pH from 6.8 to 6.5. 

 Sterilization: Not specified. 



Use: To study behavior of the colon aero- 

 genes group with dyes. Author reported 

 that the more basic the medium the more 

 of the basic dye was absorbed by bac- 

 terial protein. 



Variants: The author added 2.0 g. lactose. 



Reference: Stearn and Stearn (1923 p. 568). 



523. Rivas' Trypsinized Peptone Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) 10.0 g. 



3. Trypsin 5.0 g. 



Preparation : 



(1) Dissolve 10.0 g. Witte's peptone with 

 gentle heating in 200-300 cc. of water. 



(2) Dissolve 5.0 g. trypsin in 10-20 cc. 

 water by shaking and heating not to 

 exceed 40°C. 



(3) Add (2) to (1). 



(4) Incubate (3) at 38-40°C. for 2 to 3 

 hours, stirring gently every 15 to 20 

 minutes. 



(5) Neutralize the reaction after the 2 or 3 

 hours if necessary. 



(6) Add water to make up to 1000.0 cc. 



(7) Boil and filter. 



(8) Distribute in tubes. 

 Sterilization: Sterilize in the steamer or 



autoclave. 

 Use: Study of indol production. 

 Variants: Norton and Sawyer adjusted the 



reaction to +1.0 to phenolphthalein. 

 References: Rivas (1912 p. 549), Roddy 



(1917 p. 42), Norton and Sawyer (1921 



p. 473). 



524. Dunham's Peptone Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. NaCl (0.5%) 5.0 g. 



3. Peptone (1.0%) 10.0 g. 



Preparation : 



(1) Autoclave 1, 2 and 3 to two atmos- 

 pheres. 



(2) Filter thru paper. 

 Sterilization: Not specified. 



Use: Detection of cholera vibrio. Author 

 reported that when concentrated H2SO4 

 was poured down the side of a tube con- 

 taining a culture of the cholera bacillus, 

 a red ring developed where the solutions 

 came together. A 5.0 or 10.0% peptone 

 solution gave better and quicker results. 



Variants : 



(a) Dunham used 5.0 or 10.0% peptone. 



(b) Smith used the medium to determine 

 indol and phenol production. 



(c) Sears used 2.0% peptone. 



(d) Myers used 3.0% Witte, Difco or 

 Fairchild peptone. Used to study 

 production of HjS. 



(e) Bezangon specified the use of Chapo- 

 teaut peptone. 



(f) Percival used 2.0% peptone. Used to 

 test for indol production. 



(g) Norton and Sawyer specified Armours 

 peptone. 



(h) Harvey used from 1.0 to 2.0% pep- 

 tone. At a pH = 8.0 to 9.0 the 

 medium was used for the isolation of 

 V. cholerae. 



(i) Lohnis and Stitt specified the use of 

 Witte's peptone. 



(j) Wherry dialyzed the peptone for 2 

 days before using in the solution. 



(k) Bristol used a 25% Difco peptone 

 solution with 0.5% NaCl to study the 

 metabolism of B. botulinus. 



(1) Almy and James used a 3.0% peptone 

 and 0.5% NaCl solution to study the 

 volatile sulphur compounds pro- 

 duced by Salmonella aertrycke and 

 Proteus vulgaris. They reported 

 that when cysteine was added to this 

 medium it was completely decom- 



References: Dunham (1887 p. 338), Smith 

 (1902 p. 94), Frost (1903 p. 64), Smith 

 (1905 p. 195), Wherry (1905 p. 439), Abel 



