148 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(1912 p. 23), Lohnis (1913, p. 44), Sears 

 (1916 p. 110), Roddy (1917 p. 42), Besson 

 (1920 p. 29), Myers (1920 p. 242), Bezan- 

 Qon (1920 p. Ill), Percival (1920 p. 113), 

 Buchan (1910 p. 108), Norton and Sawyer 

 (1921 p. 473), Harvey (1921-22 p. 101), 

 Giltner (1921 p. 42), Abbott (1921 p. 140), 

 Dopter and Sacquepee (1921 p. 119), 

 Bitfield (1922 p. 118), Stitt (1923 p. 35), 

 Park, Williams and Krumwiede (1924 

 p. 120), Cunningham (1920 p. 18), Bristol 

 (1925 p. 467), Almy and James (1926 

 p. 323). 



525. Huss' Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: To study indol production by aroma 

 producing bacteria. Bacillus esterifi- 

 cans, Maassen, and Pseudomonas Tri- 

 folii. Reaction for indol was negative 

 wlien using H2SO4 and several drops of 

 1.0% KNO2 as a test for indol. 



Variants: Brussoff used this solution to 

 cultivate iron bacteria from sludge, 

 Ferribacterium duplex. A yellow mem- 

 brane formed after 3 days. 



Reference: Huss (1907 p. 61), Brussoff 

 (1918 p. 195). 



526. Nencki's Peptone Solution 



Constituents : 



1. Water 900.0 cc. 



2. Peptone 100.0 g. 



3. NaCl 20.0 g. 



Preparation : 



(1) Dissolve 100.0 g. peptone in 900.0 cc. 

 water. 



(2) Add 20.0 g. NaCl to (1). 



(3) Filter. 



(4) Distribute into test tubes. 

 Sterilization: Sterilize in the autoclave. 

 Use: To study cause of cattle plague. 



When the medium was inoculated with 

 material containing the causative organ- 

 ism of cattle plague, round cells 1 to 3^ 

 developed after 2 days. 

 Reference: Nencki (1898 p. 530). 



527. Brussoff's Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Witte) 10.0 g. 



3. Iron peptone (Merck) 1.0 g. 



4. NaCl 10.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1 by heating in 

 streaming steam for 30 minutes. 



(2) Filter. 



(3) Adjustment of reaction not specified. 

 Sterilization: Sterilize in the autoclave 



under 1.5 atmospheres pressure for 10 



minutes. 

 Use: Cultivation of iron bacteria (sludge 



forms), Ferribacterium duplex. 

 Reference: Brussoff (1918 p. 196). 



528. Redfield's Peptone Solution (Myers) 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone (Witte or Difco) . . 300.0 g. 



3. NaCl 75.0 g. 



Preparation : 



(1) Boil 2 in 700.0 cc. tap water and 

 75.0 g. NaCl until as much of peptone 

 as possible has gone into solution. 

 (RedfieldusedKCl.) 



(2) Cool rapidly and make up to 1 liter. 



(3) Heat to boiling in a flask, plug and 

 cool rapidly. 



(4) Add 0.5% NaCl (to clear). 



(5) Filter cold thru paper. 



(6) Distribute in 150.0 cc. Erlenmeyer 

 flasks in 10.0 cc. lots. 



Sterilization: Sterilize at 15 pounds for 



15 minutes. 

 Use: Detection of H2S by bacteria from 



animal feces. Used lead acetate paper 



to detect HoS production. 

 Reference: Myers (1920 p. 232-235). 



529. Berman and Rettger's Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) 5.0 g. 



3. NaCl 5.0 g. 



Preparation: 



(1) Dissolve 2 and 3 in 1. 



(2) Tube in 10.0 cc. lots. 

 Sterilization: Not specified. 



