CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



149 



Use: To study bacterial nutrition and me- 

 tabolism. 

 Variants : 



(a) Authors used 1.0% of VVitte's pep- 

 tone. Other peptones were also 

 used. Digestive Ferments Co., Park 

 Davis, Armour, Eimer and Amend 

 and Arlington Chemical Co.'s "ami- 

 noids" were used. 



(b) Authors used 0.5% or 1.0% of one of 

 the above mentioned peptones and 

 added 0.25 g. Liebig's beef extract. 



Reference: Berman and Rettger (1918 

 pp. 371-377, 392). 



530. Migula's Peptone Solution 

 Constituents: 



1. Distilled water 600.0 cc. 



2. NaCl 0.5 g. 



3. Peptone 5.0 to 15.0 g. 



Preparation: 



(1) Add 0.5 g. of NaCl to 600.0 cc. of dis- 

 tilled water and boil for 30 minutes. 



(2) Add from 5.0 to 15.0 g. of peptone 

 to hot (1). 



(3) Filter. 



(4) Tube. 



Sterilization: Steam for 15 minutes. 



Use: Chiefly for the detection of typhoid 



and cholera bacilli. 

 Reference: Migula (1901 p. 19). 



531. Heap and Cadness' Basal Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) (3.0%). . . . 30.0 g. 



3. NaCl (0.25%) 2.5 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1 by steaming. 



(2) Filter. 



(3) Adjust to pH = 7.6. 

 Sterilization: Autoclave at 115°C. for 20 



minutes. 



Use: To study hydrogen sulphide produc- 

 tion by B. aertryke. 



Added nutrients: The authors added one of 

 the following combinations: 



(a) 2.0% glucose (sterilized by steaming 

 as a 50% solution). 



(b) 0.42% Na2HP04. 



(c) 0.42% Na2HP04 + 2.0% glucose. 



(d) 0.05% glucose. 



(e) 0.05% glucose + 0.42% NajHPOi. 



(f) 0.025% glucose. 



(g) 0.025% glucose + 0.42% NaoHPO^. 

 (h) 0.01, 0.25, 0.05 or 2.0% maltose. 



(i) 0.05, 0.1 or 2.0% xylose, 

 (j) 2.0% sucrose. 

 Variants: The authors used the basic me- 

 dium without any additions. 

 Reference: Heap and Cadness (1924-25 

 p. 80-90). 



532. Browning, Gilmore and Machle's 

 Brilliant Green Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Witte or Rostock). 20.0 g. 



3. NaCl 5.0 g. 



4. Brilliant green 



(Bayer's brilliant green extra cryst.) 

 Preparation : 



(1) Add 2 and 3 to 1. 



(2) Steam in a Koch sterilizer for J of 

 an hour. 



(3) Filter thru paper. 



(4) Distribute in 5.0 cc. lots. 



(5) Prepare a 1.0% stock solution of 

 brilliant green (Bayer's brilliant 

 green extra cryst), in distilled water. 



(6) Just before use of medium a 1:10,000 

 dilution is prepared by adding 0.1 cc. 

 of (5) to 9.9 distilled water. 



Sterilization: Sterilize at 120°C. for 15 

 minutes (medium reacts faintly alkaline 

 to litmus). 



Use: Enrichment of Bacillus typhosus. 



Variants: Harvey used 10.0 to 20.0 g. pep- 

 tone and added 25.0 cc. of a 1-10,000 

 brilliant green solution. 



References: Browning, GilmourandMackie 

 (1913-14 p. 338), Harvey (1921-22 p. 91). 



533. Harvey's Telluric Acid Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. NaCl 5.0 g. 



3. Peptone 10.0 to 20.0 g. 



4. Brilliant green (1-10,000 soln.) 



5. Telluric acid (1-1000 soln.) 

 Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Reaction of pH = 7.2. 



(3) Tube in 10.0 cc. quantities. 



(4) Add 0.1, 0.2, 0.35, 0.5 or 0.7 cc. of a 



