150 



CULTURE MEDIA' FOR CULTIVATION OF MICROORGANISMS 



freshly prepared 1:10,000 brilliant 

 green solution to each tube. 

 (5) Add 0.2 cc. of a 1:1000 telluric acid 

 solution to each tube. 

 Sterilization: Not specified. 

 Use: Enrichment medium. The inositol 



fermenters were suppressed. 

 Reference: Harvey (1921-22 p. 91). 



534. Smith's Rosolic Acid Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NaCl 5.0 g. 



3. Peptone 10.0 g. 



4. Rosolic acid (0.5% soln. in 



80.0% alcohol) 2.0 cc. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Filter. 



(3) Prepare a 0.5% rosolic acid solution 

 in 80.0% alcohol. 



(4) Add2.0cc. of (3)to(2). 



(5) Tube. 



Sterilization: Sterilize the fractional 



method. 

 Use: To detect acid and alkali production. 



Medium is pale pink. Colorless with 



acid and red with alkaline reaction. 



Enrichment of colon typhoid group. 

 Variants : 



(a) Harvey used 10.0 to 20.0 g. peptone. 



(b) Bronfenbrenner added 0.05 to 62.5 

 cc. of a 2.0% solution of Grubler 

 rosolic acid in 50.0% alcohol to the 

 peptone solution. He recommended 

 the use of 0.25% stock rosolic acid 

 solution for enrichment of the in- 

 testinal bacteria. 



References: Smith (1902 p. 94), Ball (1919 

 p. 83), Bronfenbrenner (1920 p. 84), 

 Harvey (1921-22 p. 88). 



535. Bronfenbrenner's China Blue Peptone 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NaCl (c.p.) 5.0 g. 



3. Peptone (Difco) 10.0 g. 



4. China blue 

 Preparation : 



(1) Prepare a stock solution by dissolv- 

 ing 1.0 g. of Grubler's china blue in 

 100.0 cc. of 50.0%, alcohol. 



(2) Dissolve 2 and 3 in 1. 



(3) Add 0.25% of (1) to (2). 



Sterilization: Not specified. 



Use: Enrichment of colon typhoid group. 

 Using a mixture of dyes (china blue and 

 rosalic acid). The author reported that 

 the rosolic acid was the dye that inhibited 

 the gram positive rods. 



Variants: The author prepared a stock 

 china blue and rosolic acid solution by 

 dissolving 10.0 g. Grubler's china blue 

 and 2.0 g. Grubler's rosolic acid in 

 100.0 cc. of 50.0% alcohol. This mixture 

 of dyes (0.25%) was added to the medium 

 instead of just china blue solution alone. 



Reference: Bronfenbrenner (1920 p. 184). 



536. Diedudonne's Nitrate Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KNO3 (0.01%) 0.1 g. 



3. Peptone (1.0%) 10.0 g. 



Preparation : 



(1) Prepare a 1.0%, slightly alkaline pep- 

 tone solution. 



(2) Add 0.01% KNO3 to (1). 



(3) Distribute in 10.0 cc. lots. 

 Sterilization: Method not given. 



Use: Study of nitrate reduction by colon 



typhoid group. 

 Variants: 



(a) Author used 0.001% or 0.0001% 

 KNO3. 



(b) Grimbert specified the use of Colas 

 peptone and used 1.0 g. KNO3. 



(c) Maassen used 5.0% peptone and 0.5% 

 KNO3. 



(d) Maassen used 5.0% peptone, 0.5% 

 KNO3 and added 1.0% glycerol. 



(e) Heinemann used 1.0 g. peptone and 

 added 5.0 cc. of a 2.0% KNO3 solution 

 instead of 0.1 g. KNO3. 



(f) Committee A. P. H. A. and Harvey 

 used 1.0 g. peptone and 2.0 g. KNO3. 



(g) Killer used 10.0 g. KNO3. 



(h) Rogers, Clark and Davis, Tanner, 

 Ball, Giltner, and Park, Williams 

 and Krumwiede used 1.0 g. peptone 

 andO.2g.KNO3. 



(i) Committee S. A. B. used 2.0 to 5.0 g. 

 peptone and 2.0 to 5.0 g. KNO3. 



(j) Tanner used 1.0 g. Witte's peptone 

 and2.0g. KNO3. 



(k) Conn and Breed used 0.2 or 2.0 g. 

 nitrate. 



