CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



151 



(1) Conn and Breed used 0.2 or 2.0 g. 



nitrate and added 10.0 g. glucose. 

 (m) Conn and Breed used 0.2 g. KNO3 



with 2.0 or 5.0 g. peptone, 

 (n) Conn and Breed used 0.2 g. KNO3, 



2.0 or 5.0 g. peptone and added 



10.0 g. glucose. 

 References: Diedudonne (1894-95 p. 510), 

 . Grimbert (1898 p. 385), Maassen (1902 

 p. 28), Heinemann (1905 p. 131), Com- 

 mittee A. P. H. A. (1905 p. 109), Killer 

 (1913 p. 521), Rogers, Clark and Davis 

 (1914 p. 415), Committee S. A. B. (1918 

 p. 116), Tanner (1919 p. 90), Conn and 

 Breed (1919 pp. 273-275), Tanner (1919 

 p. 45), Ball (1919 p. 76), Giltner (1921 

 p. 42), Harvey (1921-22 pp. 102, 108), 

 Park, Williams and Krumwiede (1924 

 p. 124). 



537. Harvey's Nitrate Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NaNO, 1.0 g. 



3. Peptone 10.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: Study of nitrate reduction. 

 Reference: Harvey (1921-22 p. 107). 



538. Cohen and Clark's Phosphate Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Dif co) 10.0 g. 



3. K2HPO4 5.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Adjust to any desired pH value by 

 the addition of HCl or NaOH. 



Sterilization: Not specified. 



Use: To study effect of reaction (pH) on 

 growth of the colon-aerogenes group, and 

 to study ammonification. 



Variants: Cunningham studied ammoni- 

 fication using the following soil and 

 manure forms, Bac. mycoides, Bad. 

 fluorescens, Bad. vulgar e, Bad. coli, 

 Sarcina lutea, Bac. putrificus. He speci- 

 fied the use of tap water and used 0.05% 

 K2HPO4 instead of 0.5%. 



Reference: Cohen and Clark (1919 p. 410). 



539. Enlow's Basal Agar Peptone 

 Solution 

 Constituents : 



1. Water (tap or distilled) 2000.0 cc. 



2. Peptone 10.0 g. 



3. K2HPO4 17.0 g. 



4. Agar 2.0 g. 



5. Indicator (Brom thymol blue) 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1800.0 cc. 

 water in the Arnold sterilizer by 

 heating for 40 minutes or autoclave 

 for 10 minutes or autoclave for 10 

 minutes at 15 pounds pressure. 



(2) Filter through paper while hot. 



(3) Dilute to 2000.0 cc. by the addition 

 of hot distilled water. 



(4) Adjust the hydrogen ion concentra- 

 tion as desired. 



(5) Add 0.5% of one of the added nu- 

 trients. 



(6) Add any desired indicator. (The 

 author added 3 drops of Brom thymol 

 blue to each 500.0 cc. of medium. 

 The indicator solution was prepared 

 by grinding 0.1 gram Brom thymol 

 Blue and 3.2 cc. of N/20 NaOH in an 

 agate mortar. When solution of the 

 dye was complete, add 3.0 cc. of dis- 

 tilled water. 



(7) Tube. 



Sterilization: Sterilize for 30 minutes in 

 the Arnold sterilizer on each of 3 succes- 

 sive days. 



Use: Used as a basic sugar free medium. 



Variants : The author used 14.5 g. Na2HP04 

 and 1.4 g. KH2PO4 instead of 17.0 g. 

 K2HPO4. The combination of Na2HP04 

 and KH2PO4 gave a medium with a pH 

 of between 7.2 and 7.4. 



Added nutrients: The author added 0.5% 

 of any desired sugar, alcohol, glucose, etc. 



Reference: Enlow (1923, p. 2130). 



540. Beijerinck's Ammonium Sulphate 

 Peptone Solution 

 Constituents: 



1. Water (ditch) 1000.0 cc. 



2. Peptone 5.0 g. 



3. (NH4)2S04 1.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in ditch water 

 ("Grabenwasser ' ' ) . 



(2) Make alkaline with NsaCO,. 



