152 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Sterilization: Not specified. 



Use: To study reduction of sulphates by 

 Spirillum tenue, generally the more 

 organic material that is present the 

 greater the reduction. 



Variants : Cathelineau studied pigment and 

 fluorescent production by Bacillus varidis 

 (Lesage) and used Beijerinck's solution 

 with one of the following: 



glucose 5.0 g. 



sodium phosphate 5.0 g. 



sodium succinate 5.0 g. 



Reference: Beijerinck (1895 p. 107), Cathe- 

 lineau (1896 p. 235). 



541. Redfield's Peptone Solution (Tanner) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone 300.0 g. 



3. KCl 74.0 g. 



Preparation : 



(1) Add 2 and 3 to hot 700.0 cc. distilled 

 water. 



(2) Stir until solution is complete. 



(3) Cool. 



(4) Allow to stand in the ice box over 

 night. 



(5) Filter. 



(6) Make up to one liter. 



(7) Tube. 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Tanner (1919 p. 59). 



542. NicoUe, Raphael and Debaens Peptone 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Defresne) 25.0 g. 



3. Soda (normal) 20.0 cc. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Tube in 4.0 cc. lots. 

 Sterilization: Sterilize at 110°C. for 15 



minutes. 



Use: Cultivation of Eberth's bacillus and 

 paratyphoid bacilli. Indol was not pro- 

 duced in this medium. 



Reference: NicoUe, Raphael and Debains 

 (1917 p. 380). 



543. Levine's Boric Acid Peptone Solution 



Constituents : 



1. Water 



2. Peptone (1.0%) 10.0 g. 



3. Boric acid (H3BO3 0.63%) . . 6.3 g. 

 Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 

 Use: To determine the efltect of boric acid 



on the growth of Bad. coli and Bad. 

 aerogenes. The author reported that 

 Bad. aerogenes died off in this medium 

 but that Bad. coli multiplied slowly. 

 Reference: Levine (1911 p. 22). 



544. DeBord's Phosphate Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Dif co) 32.0 g. 



3. KH2PO4 (M/2 solution) .... 16.0 cc. 



4. NajHPOi (M/2 solution) . . . 144.0 cc. 

 Preparation : 



(1) Dissolve peptone in 900.0 cc. cold 

 distilled water. 



(2) Add 16.0 cc. half molar KH2PO4 and 

 144.0 cc. of half molar Na2HP04 

 solutions. 



(3) Dilute to 1500.0 cc. 



(4) Heat for 15 minutes at 15.0 pounds 

 pressure. 



(5) Filter and make up loss in weight. 



(6) Add 100.0 cc. distilled water. 

 Sterilization: Sterilize to 15 pounds pres- 

 sure for 30 minutes. 



Use: To study nitrogen metabolism of B. 

 coli, Ps. pyocyanea, C. sporogenes and 

 P. subtihs. 



Variants: The author added 100.0 cc. of a 

 sterile glucose solution prepared by 

 dissolving 16.0 g. glucose in distilled 

 water instead of the 100.0 cc. distilled 

 water. 



Reference: DeBord (1923 pp. 16, 17). 



546. Kendall, Day and Walker's Fat Free 

 Peptone Solution 



Constituents : 



1. Distilled water • 1000.0 cc. 



2. Peptone (Fairchilds) 15.0 g. 



3. Na2HP04 3.0 g. 



4. NaCl 5.0 g. 



Preparation : 



(1) Extract 15.0 g. of 2 for two weeks with 

 ether, for two weeks with alcohol, 

 two weeks with acetone and 10 days 

 with petroleum ether, respectively, 

 in a Soxhlet extractor, the successive 

 1000.0 cc. extractions occurring at intervals of 



