CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



153 



about 12 minutes. These extractions 

 to be continued for 6 hours a day and 

 6 days per week. 



(2) Dissolve residue from the extraction 

 in 1. 



(3) Dissolve 3 and 4 in (2). 



(4) Distribute in 100.0 cc. lots. 



(5) Adjustment of reaction not given. 

 Sterilization: Autoclave (time not given). 

 Use: To study metabolism of tubercle 



bacilli. Authors used alizarin, neutral 

 red, and phenolphthalein to study changes 

 in reaction. Ziehl-Neelsen stain for 

 staining. 



Variants: Harvey used 30.0 g. of peptone 

 (did not specify Fairchilds) and added 

 0.1 cc. of 1.0% neutral lead acetate to each 

 10.0 cc. quantity of medium. 



References: Kendall, Day andjWalker (1914 

 p. 424), Harvey (1921-22 p. 107). 



547. Kligler's Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Witte's) 10.0 g. 



3. K2HPO4 0.29 g. 



4. NaCl 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjustment of reaction not given. 



(3) Filter thru filter paper. 



(4) Tube in 10.0 cc. lots. 

 Sterilization: Autoclave (time not given). 

 Use: To study indol production. 

 Reference: Kligler (1914 p. 82). 



548. Klecki's Basal Ammonium Phosphate 

 Peptone Solution 



Constituents: 



1. Water 5000.0 cc. 



2. Peptone 10.0 g. 



3. Potassium phosphate — 0.29 g. 



4. Ammonium phosphate. . . 0.5275 g. 



5. MgS04 .' 0.275 g. 



6. (NH4)2S04 0.115 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 100.0 g. of 

 one of the added nutrients in 1. 



(2) Distribute in Pasteur fermentation 

 tubes. 



Sterilization: Method not given. 



Use: Cultivation of B<tcillus sncchnro- 

 butyricus. Organism did not grow using 

 calcium lactate as a carbon source. 



Added nutrients : The author added 100.0 g. 



of lactose or 100.0 g. of calcium lactate. 

 Reference: Klecki (1896 p. 255). 



549. Pergola's Nitrate Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 10.0 g. 



4. NaaCOs 0.2 g. 



5. KNO3 0.1 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Filter. 



(3) Distribute in 15 to 20.0 cc. lots. 

 Sterilization: Method not given. 

 Use: Enrichment of cholera vibrio. 

 Variants : 



(a) Wolfel used 2.0% peptone, 0.5% NaCl, 

 0.0075% KNO3 and 0.2 g. NasCOs in 

 100.0 cc. distilled water to demon- 

 strate the cholera red reaction. 



(b) Ball used 10.0% peptone, 0.1% KNO3 

 and 0.1% NajCO,. 



References: Pergola (1911 p. 85), Wolfel 

 (1912 p. 416), Ball (1919 p. 83). 



•556. Klimmer's Nitrate Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Witte) 100.0 g. 



3. NaCl 100.0 g. 



4. KNO3 1.0 g. 



5. NaoCOa (crystalline) 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1000.0 cc. 

 distilled water. 



(2) Tube or flask. 

 Sterilization: Method not given. 



Use: Enrichment of cholera vibrio. Add 

 9 parts of water under investigation to 

 1 part of medium. 



Reference: Klimmer (1923 p. 221). 



551. Bleisch's Nitrate Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Witte) 20.0 g. 



3. NaCl (c.p.) 5.0 g. 



4. KNO3 (c.p.) 0.8 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Boil. 



(3) Filter. 



