154 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Sterilization: Method not given. 



Use: Study of indol production by cholera 



bacteria. 

 Variants : 



(a) Frost and Johnson used 1.0 g. Merck's 

 peptone, 0.2 g. KNO3 and 0.5 g. NaCI. 



(b) Wherry used 10.0 g. of Witte's pep- 

 tone and 0.01% KNO3. 



(c) Wherry used 10.0 g. of Witte's pep- 

 tone that was dialysed for two days 

 and 0.01% KNO3. 



(d) Logie used 10.0 g. peptone and 

 0.00283 g. KNO3. 



(e) Cunningham used 10.0 g. peptone, 

 5.0 g. NaCI and 0.5 g. nitrite free 

 KNO3. 



Reference: Bleisch (1893 p. 112), Frost 

 (1903 p. 66), Wherry (1905 p. 439), Logie 

 (1919 p. 147), Johnson (1912 p. 219), 

 Cunningham (1924 p. 18). 



552. Logie's Nitrite Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCI 5.0 g. 



4. NaNO. 0.0023g. 



Preparation: (1) Dissolve 2, 3 and 4 m 1. 

 Sterilization: Not specified. 



Use: To study the formation and destruc- 

 tion of nitrite by the dysentery bacilli. 

 Reference: Logie (1910 p. 147). 



553. Gottheil's Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2 Potassium phosphate 10 g. 



3.CaCU O.lg. 



4. MgS04 0.3 g. 



5. NaCI O.lg. 



6. Iron t^^«« 



7. Peptone 10-0 g- 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



inl. 



Sterilization: Not specified. 



Use: General culture medium for organ- 

 isms found in the soil, on roots and 

 rhizomes. 



Reference: Gottheil (1901 p. 432). 



554. Guyenot's Peptone Solution 



Constituents: 



1 Redistilled water 1000.0 cc. 



2. NaaS04 0.16g. 



3. CaHP04 0.32 g. 



4. MgCl, 0.56 g. 



5. KH2PO4 4.16 g. 



6. Peptone (Chapoteaut) .... 20.0 g. 

 Preparation : 



(1) Add 2, 3, 4 and 5 to 1. 



(2) Add sufficient acetic acid to keep the 

 materials in solution. 



(3) Dissolve 6 in (2). 

 Sterilization: Not specified. 



Use: Cultivation of Drosophila ampe- 



lophila. 

 Reference: Guyenot (1914 p. 548). 



555. Will's Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. CaHP04 0.5 g. 



3. KH2PO4 4.55 g. 



4. MgS04 2.1 g. 



5. Witte peptone 20.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of non-spore forming 



yeast. 

 Variants : 



(a) Will used 4.55 g. K2HPO4 instead of 

 KH2PO4 to cultivate mycoderma. 



(b) Nakozawa added 5.0% sucrose. 

 Reference: Will (1908 p. 387), (1901 p. 3), 



Nakazawa(1909p. 530). 



556. Gersbach's Trypsinized Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) 10-0 g. 



3. NaCI 0.5 g. 



4 Calcium phosphate 0.2 g. 



5. MgS04 0.02 g. 



Preparation: 



(1) Prepare a 1.0% solution of Witte's 

 peptone and add 0.5% NaCI to the 

 liter. 



(2) Boil one hour and cool to 40°C. 



(3) Neutralize and make slightly alkaline 

 with soda. 



(4) Place in flask with a well fitting glass 

 stopper. 



(5) Add 0.2 g. of Griibler's trypsin, 0.2 g. 

 calcium phosphate 0.02 f. MgS04, 

 10.0 cc. chloroform and 10.0 cc. of 

 toluol. 



