CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



155 



(6) Shake thoroly, removing the stopper 

 occasionally. 



(7) Place a paper around the flask and 

 place in the incubator at 37°C. for 

 24 hours. 



(8) Shake thoroly. 



(9) Filter thru a sterile moistened filter 

 paper. 



Sterilization: Sterilize for 45 minutes 



(method not given). 

 Use: Study of indol production. Culture 



medium used in water analysis. 

 Reference: Gersbach (1922 p. 146). 



557. Kappen's Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone (0.5%) 5.0 g. 



3. K2HPO4I 



4. NaCl I 



,- Tv,r CI/-. } usual amounts 



5. MgS04 



6. FeS04 J 



Preparation: (1) Prepare a 0.5% solution 

 of peptone in water containing the usual 

 amounts of K2HPO4, NaCl, MgSOi, and 

 FeS04 (amounts not given). 



Sterilization: Method not given. 



Use: To study decomposition of peptone 

 by cyanamide decomposing bacteria. 



Reference: Kappen (1909 p. 395). 



558. Percival's Ammonium Sulphate 

 Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. (NH4)2S04 2.0 g. 



3. K2HPO4 1.0 g. 



4. NaCl 2.0 g. 



5. MgS04 0.5 g. 



6. FeS04 0.4 g. 



7. MgC03 10.0 g. 



8. Peptone (0.01%) 0.1 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Distribute in 50.0 cc. into conical 

 flasks. 



(3) Add 0.5 g. of basic MgCOj to each 

 flask. 



(4) Add 0.01% peptone to each flask. 

 Sterilization: Not specified. 



Use: To study nitrification. 



Variants: The author used 0.03, 0.3, 1.0 or 



3.0% peptone instead of 0.01%. 

 Reference: Percival (1920 p. 144). 



559. Fremlin's Ammonium Sulphate 

 Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. (NH4)2S04 1.0 g. 



3. Potassium phosphate. 1.0 g. 



4. MgCOa 10.0 g. 



5. Peptone 0.1 to 0.5 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Dissolve 4 in the remainder of 1. 



(3) Mi.x sterile (1) and (2) under aseptic 

 conditions and add from 0.1 to 0.5 g. 

 peptone. 



Sterilization: Method not given. 



Use: To study nitrogen o.xidation by 



nitroso bacteria. 

 Reference: Fremlin (1903 p. 368). 



560. Mortensen's Peptone Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte (1.0%)) .... 10.0 g. 



3. Cobalt salts 

 Preparation : 



(1) Dissolve 2 in 1. 



(2) Add cobalt salts in varying amounts 

 from 4 to 1/32%. 



Sterilization: Not specified. 



Use: To study the effect of cobalt salts on 

 Aspergillus niger. The author reported 

 that the toxic properties of cobalt were 

 different in different media. A 0.5% 

 cobalt chloride solution in a liquid 

 medium was as toxic as 1.0% cobalt 

 chloride in a gelatin medium. 



Reference: Mortensen (1909 p. 523). 



561. Kligler's Nitrate Peptone Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone 10.0 g. 



3. K2HPO4.. 0.2 g. 



4. MgS04 0.1 g. 



5. NaCl 0.2 g. 



6. KNO3 0.2 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization: Not specified. 



Use: To study denitrification by cocci. 

 Author reported that cocci reduced 

 nitrates to nitrites only. Ammonia was 

 formed but as a decomposition product 

 of the peptone. 



