156 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Variants: The KNO3 was omitted. 

 Reference: Kligler (1913 p. 790). 



562. Percival's Phosphate Peptone Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. KH2PO4 2.0 g. 



3. Magnesium phosphate 0.1 g. 



4. Peptone 10.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 4 to (1). 



(3) Heat to boiling. 



(4) Filter. 



(5) Adjustment of reaction not specified. 



(6) Tube in 10 to 15.0 cc. quantities. 



(7) After sterilization inoculate with con- 

 tents of a nodule of a pea, runner 

 bean, red clover, and broad bean by 

 means of a sterile knife. 



Sterilization: Sterilize on 3 successive days 



(time not specified). 

 Use: Cultivation of bacteria from the 



nodules of legumes. 

 Reference: Percival (1920 p. 204). 



563. Capaldi and Proskauer's Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. NaCl (0.02%) 0.2 g. 



3. MgS04 (0.01%) 0.1 g. 



4. K2HFO4 (0.2%) 2.0 g. 



5. Peptone (Witte 0.5%) 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of colon-typhoid group. 

 Variants : 



(a) The author used 2.0% peptone for 

 0.5% and used either 0.5 or 2.0% of 

 one of the following instead of 

 Witte's peptone. 



Peptone (e Carne Konig) 



Peptone (Aschman) 



Peptone (Chapoteaut) 



Peptone, driisen (Kiihne) 



Hemialbumose (Griibler) 



Somatose 



Peptone (Glutin (Paal)) 



(b) Percival used the following solution 

 to determine ammonia production 

 by B. mycoides: 



1. Water 1000.0 cc. 



2. K2HPO4 5.0 g. 



3. MgS04 2.5 g. 



4. NaCl 2.5 g. 



5. Peptone (0.1%) 1.0 g. 



References : Capaldi and Proskauer (1896 



p. 460), Percival (1920 p. 112). 



564. Ritter's Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Witte) 10.0 g. 



3. MgS04 1.0 g. 



4. K2HPO4 1.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Bacillus prodigiosus 



yeast and iron bacteria. 

 Variants : 



(a) The author added 10.0 to 20.0% of 

 glucose and specified that other 

 sugars might be added instead of 

 glucose if desired. 



(b) Zikes cultivated Cladothrix dicho- 

 toma and Cladothrix natans in the 

 following solution. 



1. Water 1000.0 cc. 



2. MgS04 1.0 g. 



3. K2HPO4 0.25 g. 



4. Peptone 1.25 g. 



He substituted a nearly ash and iron 

 free peptone or a "Manganpeptone" 

 for ordinary peptone and reported 

 slightly poorer growth using the ash 

 and iron free peptone. 



(c) Bokorny cultivated yeast in the 

 following solutions: 



1. Water 1000.0 cc. 



2. Peptone 50.0 g. 



3. KH2PO4 1.0 g. 



4. MgS04 1.0 g. 



or 



1. Water 1000.0 cc. 



2. KH2PO4 4.0 g. 



3. MgS04 1.0 g. 



4. Peptone 25.0 g. 



References: Ritter (1900 p. 207), Zikes 



(1915 p. 542), Bokorny (1917 p. 370), 

 (1920 p. 23). 



565. Speakman's Basal Peptone Solution 

 (McCoy et al.) 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. KH2PO4 0.5 g. 



